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C113S KVS-1-HA
Plasmid#113070PurposeExpresses worm KVS-1 C113S in mammalian cellsDepositorInsertC113S K+ voltage sensitive channel subunit 1 (kvs-1 Nematode)
UseTagsHAExpressionMammalianMutationC113SPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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KG#81
Plasmid#110876PurposeExpresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscleDepositorInsertsmyo-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#244
Plasmid#110927PurposeVector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cordDepositorInsertsunc-129 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#80
Plasmid#110875PurposeExpresses the C. elegans acy-1 cDNA in body wall muscleDepositorInsertsmyo-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#493
Plasmid#110932PurposeExpresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tailDepositorInsertsunc-17 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#94
Plasmid#110931PurposeExpresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tailDepositorInsertsunc-17 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#83
Plasmid#110877PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronallyDepositorInsertsrab-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP574-1
Plasmid#99477Purpose3XFlag::Topaz::MycDepositorInsert3XFlag::Topaz::Myc
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
CTL2 (M2225R)
Plasmid#96952PurposeExpresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coliDepositorInsertCTL2 (M222R) of PIEZO1
UseTags6x Histidine TagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
CTL2
Plasmid#92212PurposeExpresses C-Terminal segment of C. elegans Piezo1 in E. coliDepositorInsertCTL2 of PIEZO1
UseTags6x Histidine TagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP757-1
Plasmid#99493Purpose6XHis::TEV::3XFlag::linker::MycDepositorInsert6XHis::TEV::3XFlag::linker::Myc
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP1071-2
Plasmid#99502Purpose3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEVDepositorInsert3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP996-1
Plasmid#99499Purpose3XFlag::meGFP::linker::TEVDepositorInsert3XFlag::meGFP::linker::TEV
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP835-1
Plasmid#99495PurposeTEV::meGFP::3XFlagDepositorInsertTEV::meGFP::3XFlag
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP605-1
Plasmid#99482PurposeMyc::TEV::3XFlag::TEV::MycDepositorInsertMyc::TEV::3XFlag::TEV::Myc
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP686-1
Plasmid#99491PurposeTEV::eGFP::ER::V5::linker::3XFlagDepositorInsertTEV::eGFP::RE::V5::linker::3XFlag
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP640-2
Plasmid#99487Purpose3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlagDepositorInsert3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP1298‐1
Plasmid#87248Purposegtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP553‐1
Plasmid#87240Purposegtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)DepositorInsertgtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPICZC_CeTRIC-B2_Del
Plasmid#87345PurposeInducible expression of CeTRIC-B2 in P.Pichia strainsDepositorInsertTRIC-B2
UseTagspolyhistidineExpressionYeastMutationΔ253-313PromoterAOX1Available sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBN279
Plasmid#86725PurposeTissue-specific FLP expression plasmid for C. elegansDepositorInsertPhlh-8::FLP D5
UseTagsExpressionWormMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
TU#1412_tiam-1_cDNA
Plasmid#82364Purposeexpress tiam-1 cDNADepositorInserttiam-1 cDNA (tiam-1 Nematode)
UseTagsExpressionWormMutationPromoternoneAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-lin26
Plasmid#80357PurposePmex-5:GFP::H2B::PEST::lin-26 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::lin-26 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-atg4.2
Plasmid#80352PurposePmex-5:GFP::H2B::PEST::atg4.2 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::atg4.2 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-set6
Plasmid#80362PurposePmex-5:GFP::H2B::PEST::set-6 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::set-6 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-ets4
Plasmid#80355PurposePmex-5:GFP::H2B::PEST::ets-4 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::ets-4 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mex3
Plasmid#80361PurposePmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::mex-3 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mbk2
Plasmid#80360PurposePmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::mbk-2 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
TU#1805_CRISPR_unc-73_exon2
Plasmid#82359Purposeto create unc-73B null alleleDepositorInsertCas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
TU#1449_unc-73e_cDNA
Plasmid#82363Purposeexpress unc-73e cDNADepositorInsertunc-73 e isoform (unc-73 Nematode)
UseTagsExpressionWormMutationPromoternoneAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJL1702
Plasmid#78195Purposeoptogenetic activation of IL2 neuron in wormsDepositorInsertChR2(gf)
UseTagsExpressionWormMutationPromoterlag-2Available sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pSR13
Plasmid#69155Purposeread-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr IIDepositorInsertsUseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0, codon-optimzed index 1.…Promoterrps-27 and swsn-1Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR11
Plasmid#69154Purposeread-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IVDepositorInsertsUseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterfzr-1 and rps-27Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKPY737
Plasmid#72673PurposeEncodes Thr412Gly-CePheRS Alpha Subunit (Gateway Entry Clone)DepositorInsertThr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
UseGateway entry cloneTagsExpressionMutationThr412Gly in CePheRS Alpha SubunitPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGC461
Plasmid#71363Purposedistal tip cell expression of DAF-16 in C. elegansDepositorInsertdaf-16 (daf-16 Nematode)
UseTagsGFP(65C)ExpressionWormMutationPromoterBlelloch et al., 1999; Mathies et al., 2003Available sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc'-cYAP-1
Plasmid#66854PurposeExpress Myc-tagged C.elegans YAP-1 in mammalian cellsDepositorInsertYAP-1 (yap-1 Nematode)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc'-EGL-44
Plasmid#66855PurposeExpress Myc-tagged C.elegans EGL-44 in mammalian cellsDepositorInsertEGL-44 (CELE_F28B12.2 Nematode)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pClneoEGFPC-let60 K16N
Plasmid#66861PurposeExpress GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cellsDepositorInsertLet-60 (let-60 Nematode)
UseTagsGFPExpressionMammalianMutationLysine 16 is mutated to asparaginePromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD95.75 RSF-1
Plasmid#66863PurposeGenerate transgenic C..elegans expressing GFP-fused RSF-1DepositorInsertRSF-1 (rsf-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-LIN-29-pcDNA3.1-
Plasmid#52515Purposeexpresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertLin-29 b (lin-29 Nematode)
UseTagsRGS-6xHisExpressionMammalianMutationwt (changed coding sequence in 5' (5th codon…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT mir-1-1 reporter cel-mir-240
Plasmid#46742DepositorInsertcel-mir-240
UseRNAiTagsHis and hsa-mir-1-1ExpressionInsectMutationPromoterMetallothionein (MT)Available sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT mir-1-1 reporter cel-mir-230
Plasmid#46741DepositorInsertcel-mir-230
UseRNAiTagsHis and hsa-mir-1-1ExpressionInsectMutationPromoterMetallothionein (MT)Available sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT mir-1-1 reporter cel-mir-50
Plasmid#46740DepositorInsertcel-mir-50
UseRNAiTagsHis and hsa-mir-1-1ExpressionInsectMutationPromoterMetallothionein (MT)Available sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only
