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pLIC-lyn11-myc-LOV2GIVe (L)

Plasmid

#170624

Purpose

For the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539E

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (L)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe (D)

Plasmid

#170625

Purpose

For the mammalian expression of mLOV2GIVe dark-mimicking mutation C450S

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (D)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-GRK2RH-Nluc

Plasmid

#158605

Purpose

Plasmid expressing GRK2(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn-Gβγ-BERKY1

Plasmid

#158429

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-GRK3ct

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB-1753-Nluc

Plasmid

#158603

Purpose

Plasmid expressing KB-1753-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Gαi*-BERKY3

Plasmid

#158424

Purpose

BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Use

Lentiviral

Tags

myc

Expression

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn-Gαi*-BERKY3

Plasmid

#158428

Purpose

BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn-Gαq*-BERKY1

Plasmid

#158430

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-GRK2(RH)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-hSyn-Gαi*-BERKY1

Plasmid

#158427

Purpose

BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-KB1753-myc

Use

Lentiviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIC-lyn11-myc-LOV2GIVe (L) (FA)

Plasmid

#170627

Purpose

For the mammalian expression of mLOV2GIVe lit-mimicking mutation I539E and the GEF-deficient mutation F1685A

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (L) (FA)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-PRGRH-Nluc

Plasmid

#158608

Purpose

Plasmid expressing PRG(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-p115RH-Nluc

Plasmid

#158607

Purpose

Plasmid expressing p115(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

Plasmid

#158604

Purpose

Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-GRK2RH-Nluc L118A

Plasmid

#158606

Purpose

Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0384

Plasmid

#141599

Purpose

Lentiviral vector for overexpressing the SMARCA2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMARCA2 (SMARCA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0383

Plasmid

#141598

Purpose

Lentiviral vector for overexpressing the SMARCA2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMARCA2 (SMARCA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0379

Plasmid

#141597

Purpose

Lentiviral vector for overexpressing the SMARCA2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMARCA2 (SMARCA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0389

Plasmid

#141600

Purpose

Lentiviral vector for overexpressing the SMARCA4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMARCA4 (SMARCA4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lp-BFPnls-LP-Cherry-T2A-NTR2.0

Plasmid

#219785

Purpose

Used for generating a transgenic line for inducing NTR2.0 expression in Cre positive cells after conversion

Depositor

Prayag Murawala

Article

García-García et al bioRxiv 2025.03.30.645595; doi: 10.1101/

Insert

mTagBFP2

Use

Tags

3x SV40 NLS

Expression

Mutation

Promoter

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-GINIP-Nluc

Plasmid

#223556

Purpose

Plasmid expressing GINIP-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

human GINIP - linker(GGGS) - Nluc

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge