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AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

Plasmid

#163476

Purpose

Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

iCre

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

Plasmid

#164458

Purpose

AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minCMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ai139 (TIT2L-GFP-ICL-TPT) targeting vector

Plasmid

#114426

Purpose

Target a Cre-dependent GFP and a tdTomato-P2A-tTA2 expression cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP, tdTomato, tTA2,

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector

Plasmid

#114433

Purpose

Target a Cre-dependent GCaMP6s cassette and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6s, tTA2

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Calb1-T2A-dgCre targeting vector

Plasmid

#114438

Purpose

Target dgCre into the endogenous mouse Calb1 locus at the stop codon

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

dgCre

Use

Mouse Targeting

Tags

DHFR domain and EGFP

Promoter

none; utilizes endogenous Calb1 promoter for expr…

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

Plasmid

#114428

Purpose

Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6f

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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AiP12014-pAAV-AiE2004m-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2014)

Plasmid

#164457

Purpose

AiE2004m is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minCMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector

Plasmid

#114430

Purpose

Target a Cre-dependent GCaMP6s cassette and a tdTomato-P2A-tTA2 cassette to the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6s, tdTomato, tTA2

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai140 (TIT2L-GFP-ICL-tTA2) targeting vector

Plasmid

#114427

Purpose

Target a Cre-dependent GFP and a tTA2 expression cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP, tTA2

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

PGK, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai133 (TITL-ssAPEX2tm) targeting vector

Plasmid

#114432

Purpose

Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ssAPEX2tm

Use

Cre/Lox and Mouse Targeting

Promoter

TREtight

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai167 (TIT2L-ChrimsonR-tdT-ICL-tTA2) targeting vector

Plasmid

#114431

Purpose

Target a Cre-dependent ChrimsonR-tdTomato cassette and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ChrimsonR-tdTomato, tTA2

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai170 (TIT2L-ASAP2s-Kv-ICL-tTA2) targeting vector

Plasmid

#114435

Purpose

Target a Cre-dependent voltage sensor ASAP2s cassette into the mouse TIGRE locus

Depositor

Michael Lin, Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ASAP2s-Kv, tTA2

Use

Cre/Lox and Mouse Targeting

Tags

Kv2.1 tag

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai161 (TIT2L-GFP-ICR-tTA2) targeting vector

Plasmid

#114429

Purpose

Target a Cre-dependent GFP cassette and Dre-dependent tTA2 cassette to the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCis mCherry-SS Himar1 A7

Plasmid

#84687

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Article

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCis GFPuv-SS Himar1 A7

Plasmid

#84686

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Article

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851)

Plasmid

#164450

Purpose

AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Has Service

AAV PHP.eB

Insert

iCre

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgH (Merlin)

Plasmid

#136448

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgH (TB40/E)

Plasmid

#136446

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1 C7G HA

Plasmid

#8954

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Mutation

C7G: point mutation in LxCxE motif, LxghE

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-HCMVgH-8his (Merlin)

Plasmid

#136447

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Tags

Short GGSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular domain (a.a. 1-716). Codon-optimize…

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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