We narrowed to 2,150 results for: Xpa

1. pCK671.306

   Plasmid

   #192641

   Purpose

   Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   J306 scRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23119

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAL1

   Plasmid

   #197285

   Purpose

   Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

   Depositor

   Bogumil Karas

   Article

   Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

   Insert

   Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
   

   Use

   Synthetic Biology; Bacterial and yeast cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB9337 (pgRNA_IV-1_NatMX)

   Plasmid

   #161590

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFP.R400

   Plasmid

   #138175

   Purpose

   Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Lyn-GR-AKAR3

   Plasmid

   #173016

   Purpose

   Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Lyn-GR-AKAR3
   

   Use

   Tags

   EGFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC-Ura-Cen-lvl1

   Plasmid

   #176182

   Purpose

   preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-Pitx2

   Plasmid

   #60668

   Purpose

   PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

   Depositor

   James Thomson

   Article

   Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

   Insert

   Pitx2 (Pitx2 Mouse)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS B2rescue A70D

   Plasmid

   #78658

   Purpose

   Generates B2 inserts suitable for rescue

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

   Insert

   B2rescue cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFP.R375

   Plasmid

   #138245

   Purpose

   Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTorPE-G-GECO1.1

    Plasmid

    #32467

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.1
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

    Expression

    Bacterial

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHJ3: LexA-VHH-Control

    Plasmid

    #108241

    Purpose

    LexA DNA binding domain fusing with anti-RNase A VHH antibody

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    LexA DNA binding domain fusing with anti-RNase A VHH antibody
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO1

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL59-Nat

    Plasmid

    #140465

    Purpose

    CRISPR/Cas9 engineering with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pILGFP3A2 (2)

    Plasmid

    #185852

    Purpose

    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEAST2-sfGFP

    Plasmid

    #126575

    Purpose

    Strong constitutive expression of super-folder GFP for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-MCS-YFP-NLS

    Plasmid

    #145274

    Purpose

    Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    MCS and YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBM13-sd4U

    Plasmid

    #179691

    Purpose

    Bacterial expression of base editor

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q1310R, S1330I, T1380I

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCNL-N1_PTS2

    Plasmid

    #65676

    Purpose

    Expresses PTS2-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    peroxisomal targeting signal 2
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFP.R271

    Plasmid

    #138220

    Purpose

    Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pILGFP9E3

    Plasmid

    #185842

    Purpose

    Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYNL-C1_H2B

    Plasmid

    #65702

    Purpose

    Expresses YNL-H2B in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    histone H2B
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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