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pAG415Gal_Gcn4 SATtoG KtoRHA

Plasmid

#232994

Purpose

Galactose iduced expression of Gcn4 SATtoG KtoRHAin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 SATtoG KtoR

Plasmid

#232961

Purpose

Galactose iduced expression of Gcn4 ATtoG KtoR in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 SATtoG KtoRGFP

Plasmid

#233006

Purpose

Galactose iduced expression of Gcn4 SATtoG KtoRGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 solvvol W

Plasmid

#232977

Purpose

Galactose iduced expression of Gcn4 solvvol W+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 SATtoG KtoR

Plasmid

#231861

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoR

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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FAM177A1-Halo

Plasmid

#224591

Purpose

For expression of FAM177A1-Halo in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Tags

HALO

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED solvvol

Plasmid

#231858

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED solvvol

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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ZFPL1-GFP

Plasmid

#224590

Purpose

For expression of ZFPL1-GFP in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

ZFPL1 (ZFPL1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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FAM177A1-SNAP

Plasmid

#224592

Purpose

For expression of FAM177A1-SNAPTag in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Tags

SNAP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI3-3×FLAG

Plasmid

#215511

Purpose

Expresses FLAG tagged human WIPI3.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 3 (WDR45B Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DXO

Plasmid

#177992

Purpose

For gateway cloning of DXO

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

DXO (DXO Human)

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-WT

Plasmid

#215897

Purpose

Expression of TNRC6A-silencing domain (SD)

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT

Plasmid

#215898

Purpose

Expression of TNRC6A-CIM1 domain

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT

Plasmid

#215899

Purpose

Expression of TNRC6A-CIM2 domain

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1529-1709

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A

Plasmid

#215900

Purpose

Expression of TNRC6A-CIM1 domain with S1332A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528, S1332A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1346A

Plasmid

#215901

Purpose

Expression of TNRC6A-CIM1 domain with S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528, S1346A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTE5418_pMBP-GlcR

Plasmid

#221192

Purpose

Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Insert

glcR (pden4400 from Paracoccus denitrificans)

Tags

10x His tag with E. coli maltose binding protein …

Expression

Bacterial

Promoter

T7 promoter and T7 promoter-lacO

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP9A-HA

Plasmid

#209230

Purpose

Mammalian expression of ATP9A

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9A (ATP9A Human)

Use

Retroviral

Tags

HA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

Plasmid

#223163

Purpose

2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3_C_CCDC22(Y557C)

Plasmid

#209695

Purpose

Expresses MAC3-tagged CCDC22(Y557C) in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

CCDC22 (CCDC22 Human)

Tags

MAC3-tag

Expression

Mammalian

Mutation

Y557C, changed Tyrosine 557 to Cysteine

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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