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We narrowed to 17,816 results for: multi

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Showing: 6381 - 6400 of 17816 results

  3. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120813-plasmid-map-sequence-id-235308

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  6. pRS416gT-GalL-Cas9

    Plasmid
    #79903
    Purpose
    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression
    Depositor
    Ronald Davis
    Article
    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw
    Inserts
    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GPM1 promoter, GalL, and RPR1 promoter with TetO …
    Available Since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    79903-plasmid-map-sequence-id-157872

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  7. pCMV/MASC_(GLuc)_INT

    Plasmid
    #68440
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    68440-plasmid-map-sequence-id-124660

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  11. AAV-DIO-TC66T-2A-EGFP-2A-oG

    Plasmid
    #178429
    Purpose
    Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950 Glu66→Thr
    Optimized G
    EGFP
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Promoter
    hSyn
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178429-plasmid-map-sequence-id-354737

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  13. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Available Since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162469-plasmid-map-sequence-id-350377

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  15. pJK511

    Plasmid
    #155384
    Purpose
    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155384-plasmid-map-sequence-id-309004

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  16. pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

    Plasmid
    #115487
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    115487-plasmid-map-sequence-id-240326

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  18. pET15b LFn-Fla 3A

    Plasmid
    #84872
    Purpose
    Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Fla
    Tags
    His
    Expression
    Bacterial
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    T7
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pCru5-/GCCACC-mEGFP-IRES-mCherry

    Plasmid
    #49226
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    Viral LTR (or CMV)
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49226-plasmid-map-sequence-id-73641

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Showing: 6381 - 6400 of 17816 results