We narrowed to 9,520 results for: try

1. pYPQ292 (AaCas12b)-D570A-TV

   Plasmid

   #136380

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D570A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pG418R-GMRWhite

   Plasmid

   #165883

   Purpose

   G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Transgenesis Ready Selection Vector
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBP-ORF

   Plasmid

   #72949

   Purpose

   Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 0 - ORFs
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEnt R3L2 TetO(sh) mCh-Rs1-2

   Plasmid

   #24417

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   Rs1
   

   Use

   Entry vector

   Tags

   FLAG and mCherry

   Mutation

   Human 5HT4B receptor with D100A mutation, generat…

   Available Since

   March 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-Flag-NEDD4L∆WW2

   Plasmid

   #166708

   Purpose

   expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L W453A
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed tryptophan 453 to alanine

   Promoter

   CMV promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-Flag-NEDD4L∆WW3

   Plasmid

   #166709

   Purpose

   expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L W525A
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed tryptophan 525 to alanine

   Promoter

   CMV promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ255

   Plasmid

   #124310

   Purpose

   Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

   Insert

   rAPOBEC1-pcoCas9D10A-UGI
   

   Use

   CRISPR

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR_Hu_Plk5(WT)

   Plasmid

   #136329

   Purpose

   Human Plk5 WT in Gateway pENTR

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (PLK5 Human)
   

   Use

   Gateway entry vector

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3x-Flag Gli3 37R3-2

   Plasmid

   #120305

   Purpose

   Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C

   Depositor

   David Liu

   Article

   Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

   Insert

   Intein 37R3-2, Gli3
   

   Use

   Synthetic Biology

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains mutations as reported in 2011 Chemistry …

   Promoter

   CMV

   Available Since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR4_FLAG/HA_EWSR1_His6

    Plasmid

    #26364

    Depositor

    Thomas Tuschl

    Article

    Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Entry vector

    Tags

    FLAG/HA and His6

    Mutation

    delta 657 (stop codon)

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

11. Rae1-pDONR221

    Plasmid

    #124240

    Purpose

    Gateway entry clone of Drosophila Rae1 cDNA.

    Depositor

    Peter Vilmos

    Article

    Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

    Insert

    Drosophila Rae1 (Rae1 Fly)
    

    Expression

    Insect

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR4_EWSR1_G2A_R650H

    Plasmid

    #26372

    Depositor

    Thomas Tuschl

    Article

    Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Entry vector

    Mutation

    R650H

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pDONR223 PCSK5(M452I)

    Plasmid

    #232445

    Purpose

    Gateway pDONR vector

    Depositor

    Kevin Janes

    Article

    Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

    Insert

    PCSK5(M452I) (PCSK5 Human)
    

    Use

    Gateway entry vector

    Mutation

    M452I

    Available Since

    Nov. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDonr201 RNF146

    Plasmid

    #240330

    Purpose

    Entry vector for Gateway with RNF146

    Depositor

    Junjie Chen

    Article

    Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

    Insert

    RNF146 (RNF146 Human)
    

    Expression

    Bacterial

    Available Since

    Nov. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmRNA-iEMCV-EGFP-A120

    Plasmid

    #225905

    Purpose

    In vitro transcription of cap-independently (EMCV IRES) translated mRNA encoding EGFP.

    Depositor

    Tom Brown

    Article

    Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

    Inserts

    A-inserted T7 class III Φ6.5 promoter
    EMCV IRES
    EGFP
    2x β-globin UTR
    poly(A) tail
    

    Use

    Synthetic Biology; Mrna preparation

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmRNA-iHCV-EGFP-A120

    Plasmid

    #225906

    Purpose

    In vitro transcription of cap-independently (HCV IRES) translated mRNA encoding EGFP.

    Depositor

    Tom Brown

    Article

    Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

    Inserts

    A-inserted T7 class III Φ6.5 promoter
    HCV IRES
    EGFP
    2x β-globin UTR
    poly(A) tail
    

    Use

    Synthetic Biology; Mrna preparation

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR4-G1B3

    Plasmid

    #245067

    Purpose

    Gateway Entry vector for human G1B3 promoter

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    G1B3 promoter
    

    Expression

    Bacterial

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. mE-mCherry2A

    Plasmid

    #236248

    Purpose

    Middle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately

    Depositor

    James Amatruda, Genevieve Kendall

    Article

    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10

    Insert

    mCherry2A
    

    Expression

    Mammalian

    Mutation

    Added a viral2A sequence to mCherry with no stop …

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. mE-GFP2A

    Plasmid

    #236247

    Purpose

    Middle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately

    Depositor

    James Amatruda, Genevieve Kendall

    Article

    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10

    Insert

    eGFP
    

    Expression

    Mammalian

    Mutation

    Added a viral2A sequence with no stop codon

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR_HA-spGFP11

    Plasmid

    #240224

    Purpose

    Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Type

    Empty backbone

    Use

    Gateway shuttle vector

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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