We narrowed to 9,514 results for: try
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Plasmid#166709Purposeexpresses NEDD4L with inactivating mutation in third WW domain in mammalian cellsDepositorInsertNEDD4L W525A
TagsFLAGExpressionMammalianMutationchanged tryptophan 525 to alaninePromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ255
Plasmid#124310PurposeGateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editingDepositorInsertrAPOBEC1-pcoCas9D10A-UGI
UseCRISPRAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Hu_Plk5(WT)
Plasmid#136329PurposeHuman Plk5 WT in Gateway pENTRDepositorInsertPLK5 (PLK5 Human)
UseGateway entry vectorAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3x-Flag Gli3 37R3-2
Plasmid#120305PurposeExpresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37CDepositorInsertIntein 37R3-2, Gli3
UseSynthetic BiologyTags3X FlagExpressionMammalianMutationContains mutations as reported in 2011 Chemistry …PromoterCMVAvailable SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FLAG/HA_EWSR1_His6
Plasmid#26364DepositorAvailable SinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
Rae1-pDONR221
Plasmid#124240PurposeGateway entry clone of Drosophila Rae1 cDNA.DepositorInsertDrosophila Rae1 (Rae1 Fly)
ExpressionInsectAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_EWSR1_G2A_R650H
Plasmid#26372DepositorAvailable SinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDonr201 RNF146
Plasmid#240330PurposeEntry vector for Gateway with RNF146DepositorInsertRNF146 (RNF146 Human)
ExpressionBacterialAvailable SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmRNA-iEMCV-EGFP-A120
Plasmid#225905PurposeIn vitro transcription of cap-independently (EMCV IRES) translated mRNA encoding EGFP.DepositorInsertsA-inserted T7 class III Φ6.5 promoter
EMCV IRES
EGFP
2x β-globin UTR
poly(A) tail
UseSynthetic Biology; Mrna preparationExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmRNA-iHCV-EGFP-A120
Plasmid#225906PurposeIn vitro transcription of cap-independently (HCV IRES) translated mRNA encoding EGFP.DepositorInsertsA-inserted T7 class III Φ6.5 promoter
HCV IRES
EGFP
2x β-globin UTR
poly(A) tail
UseSynthetic Biology; Mrna preparationExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-G1B3
Plasmid#245067PurposeGateway Entry vector for human G1B3 promoterDepositorInsertG1B3 promoter
ExpressionBacterialAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
mE-mCherry2A
Plasmid#236248PurposeMiddle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separatelyDepositorInsertmCherry2A
ExpressionMammalianMutationAdded a viral2A sequence to mCherry with no stop …Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
mE-GFP2A
Plasmid#236247PurposeMiddle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separatelyDepositorInserteGFP
ExpressionMammalianMutationAdded a viral2A sequence with no stop codonAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR_HA-spGFP11
Plasmid#240224PurposeGateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.DepositorTypeEmpty backboneUseGateway shuttle vectorAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
MCUb-mVenus-pLJM
Plasmid#236789PurposeMCUB tagged with mVenus for FRET / MW change for stoichiometryDepositorAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ36-72
Plasmid#232060PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72DepositorInsertNeurofilament-Heavy tail domain lacking residues 36-72
Tags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ462-638
Plasmid#232061PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638DepositorInsertNeurofilament-Heavy tail domain lacking residues 462-638
Tags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
Tags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHtail
Plasmid#232057PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domainDepositorInsertNeurofilament-Heavy tail domain
Tags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)
Plasmid#232128PurposeA middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only