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pGWT35S-AtOEP7(1-50aa)-VN154

Plasmid

#194045

Purpose

Transient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VN154 (mVenus β-strands 1 to 7, amino acids 1−154)

Tags

AtOEP7 1–50aa

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e VPR

Plasmid

#188516

Purpose

Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-VPR

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_047

Plasmid

#180551

Purpose

Entry vector containing sfGFP in E1.3a format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP in E1.3a format

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_110

Plasmid

#180554

Purpose

Entry vector containing 7xhis tag in E1.3b format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

7xhis tag in E1.3b format

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_007

Plasmid

#180563

Purpose

Entry vector containing Terminator sequence (Bba_B0015)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator sequence (Bba_B0015)

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KBB438

Plasmid

#185092

Purpose

GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Expression

Yeast

Mutation

Rfa1 with amino acids 338-343 deleted

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB372

Plasmid

#185084

Purpose

GAL::Rfa1[330-621]-GFP under control of GAL1 promoter

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Tags

GFP

Expression

Yeast

Mutation

Rfa1 amino acids 330-621

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1 (M50P)

Plasmid

#178651

Purpose

Mammalian expression of myc-tagged MR1 (M50P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

M50P

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 MR1 (H83P)

Plasmid

#178652

Purpose

Mammalian expression of myc-tagged MR1 (H83P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

H83P

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 MR1 (Q153P)

Plasmid

#178653

Purpose

Mammalian expression of myc-tagged MR1 (Q153P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

Q153P

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_044

Plasmid

#180532

Purpose

Entry vector containing J23117 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23117 anderson promoter

Expression

Bacterial

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_043

Plasmid

#180531

Purpose

Entry vector containing J23101 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23101 anderson promoter

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_031

Plasmid

#180556

Purpose

Entry vector containing Terminator - BBa_B0010 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator - BBa_B0010 terminator

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_032

Plasmid

#180528

Purpose

Entry vector containing pLac promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pLac promoter

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_045

Plasmid

#180543

Purpose

Entry vector containing RBS 30,000 a.u. designed strength

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sRBS 30,000 a.u. designed strength

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_034

Plasmid

#180526

Purpose

Entry vector containing pLux promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pLux promoter

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_252

Plasmid

#180553

Purpose

Entry vector containing mScarlet in E1.3b format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

mScarlet in E1.3b format

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_046

Plasmid

#180544

Purpose

Entry vector containing RBS 250 a.u. designed strength

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sRBS 250 a.u. designed strength

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_042

Plasmid

#180530

Purpose

Entry vector containing J23100 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23100 anderson promoter

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mTFP1-T7

Plasmid

#173225

Purpose

Encodes mTFP fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mTFP1

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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