We narrowed to 11,748 results for: pcdna

1. pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

   Plasmid

   #228401

   Purpose

   Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1

   Depositor

   Dirk Daelemans

   Article

   Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

   Insert

   SREBP1 (SREBF1 Human)
   

   Use

   Tags

   mNeonGreen2x

   Expression

   Mammalian

   Mutation

   deleted N terminal activation domain (amino acids…

   Promoter

   EF1a

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-EGFP MASTL G44S (siRNA resistant)

   Plasmid

   #191012

   Purpose

   Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

   Depositor

   Johanna Ivaska

   Article

   Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Microtubule-associated serine/threonine kinase like (MASTL Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   G44S

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)

   Plasmid

   #40999

   Depositor

   Jonathon Pines

   Article

   Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

   Type

   Empty backbone

   Use

   Tags

   3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127321

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1(-) mouse C/EBP beta (LAP)

   Plasmid

   #12557

   Depositor

   Peter Johnson

   Insert

   CCAAT/Enhancer-binding Protein beta (Cebpb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)

   Plasmid

   #197943

   Purpose

   Expression of CoA-Snifit in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-GFP-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-G41S, D127A,L277W,F252Y

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

   Plasmid

   #164131

   Purpose

   Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-IgKappa-myc-dL5- 2XG4S-GPI

   Plasmid

   #153308

   Purpose

   Expresses GPI-anchored dL5** in mammalian cells. (FAP-GPI)

   Depositor

   Marcel Bruchez

   Article

   Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

   Insert

   IgKappa-myc-dL5-2XG4S-GPI
   

   Use

   Tags

   myc-dL5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

   Plasmid

   #44027

   Depositor

   Celeste Simon

   Article

   Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

   Insert

   mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P405A, P530V, N851A

   Promoter

   CMV

   Available since

   March 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

    Plasmid

    #226517

    Purpose

    Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

    Depositor

    Kai Johnsson

    Article

    Kühn et al bioRxiv 2024.08.28.610127

    Insert

    CalR-SNAP-tag2-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2

    Plasmid

    #205160

    Purpose

    express β-ACTIN-3′UTR-8xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Use

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3-SREBP-1-GFP (A317-318; A341)

    Plasmid

    #204198

    Purpose

    Mammalian expression of SREBP-1 (isoform 1)

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-1 (SREBF1 Human)
    

    Use

    Tags

    3xHA, eGFP and T7x2

    Expression

    Mammalian

    Mutation

    Gly to Ala 317,318,341

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1-Flag-H2A K13-15-118-119R

    Plasmid

    #63568

    Purpose

    Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine at 13, 15, 118, and 119 are now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

    Plasmid

    #100279

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Use

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Mutated catalytic domain (1025aa-End) (please see…

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

    Plasmid

    #74309

    Purpose

    Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Rat, Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. (383) pcDNA3.1-HA-nBC2-OGA(544-706)

    Plasmid

    #168100

    Purpose

    HA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nBC2-OGA(544-706)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1014 pcDNA3 T7 Akt1 K179M T308A S473A

    Plasmid

    #9031

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 K179M T308A S473A (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K179M T308A S473A

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

    Plasmid

    #49060

    Purpose

    Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP (chloride-sensitive)

    Expression

    Mammalian

    Mutation

    Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…

    Promoter

    CMV

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. 5'-3xFLAG Kdm1a-exon minimal CMV pCDNA5

    Plasmid

    #212054

    Purpose

    LR vector for integration of Kdm1a-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Kdm1a-exon (Kdm1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 His-GFP-EWSR1-myc-His

    Plasmid

    #46385

    Purpose

    expresses EGFP tagged EWSR1 in mammalian cells

    Depositor

    Heinz Gehring

    Article

    Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    EGFP, His, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only