We narrowed to 9,642 results for: Ada;
-
Plasmid#160725PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.DepositorInsertS4-ExRaiCKAR
UseAAVTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrisprV2 sgRNA hPER2c-term #1
Plasmid#179454PurposeLentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human PER2
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrisprV2 sgRNA hPER2c-term #2
Plasmid#179455PurposeLentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human PER2
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrisprV2 sgRNA hPER2c-term #3
Plasmid#179456PurposeLentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human PER2
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse Alpha2 delta-3 pMT2
Plasmid#58727Purposeexpression of mouse Alpha2 delta-3 in mammalian cellsDepositorInsertalpha2 delta-3 (Cacna2d3 Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-Phobos_CA
Plasmid#98169Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos_C128A gene
UseTagsmCherryExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…PromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-mRuby3-TEV
Plasmid#105780PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.DepositorTypeEmpty backboneUseFlp-in competentTagsmRuby3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40
Plasmid#101063PurposeAAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertCaMPARI2_F391W-G395D
UseAAVTagsFLAG-HA-myc and NES_hisExpressionMutationPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-BirA-FLAG
Plasmid#105786PurposeExpression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-BirA-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-Seipin-miniIAA7-mCherry
Plasmid#129720PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged SeipinDepositorInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-mCherryExpressionMammalianMutationsilent mutationsPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_SST1.0
Plasmid#208666PurposeExpresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in a cre-dependent mannerDepositorInsertGPCR activation based somatostatin (SST) sensor GRAB_SST1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45
Plasmid#173946PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertSOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GEF(ITSN1)
Plasmid#222631PurposeCdc42 activation using rapamycinDepositorInsertmCherry-FKBP-GEF(ITSN1) (ITSN1 Human)
UseTagsmCherry-FKBPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-EGFP
Plasmid#105793PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-EGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCriprV2-sgRNA-PER1-#1
Plasmid#189987PurposeLentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982DepositorInsertPeriod1 (PER1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27D
Plasmid#139194PurposeExpresses C-terminal HA tagged human Mfn2 S27D in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags2HAExpressionMammalianMutationSerine 27 to Aspartic AcidPromoterCMV promoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mCherry
Plasmid#105796PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB-hCRY1-mClover3-CD4-bla
Plasmid#179441PurposeDonor vector to knock in mClover3 C-terminal to human CRY1 geneDepositorInsertmClover3
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-MAPRE1
Plasmid#207794PurposeDonor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793DepositorInsertMAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5FRT/TO-Ataxin3-Strep-HA
Plasmid#113496PurposeExpression of human Ataxin-3 with C-terminal strep-HA tagDepositorInsertAtaxin-3 (ATXN3 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only