We narrowed to 9,174 results for: Pol

1. pB80-hsKIF5B(1-353)-GCN4-L-mCherry

   Plasmid

   #193715

   Purpose

   Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Human, Synthetic)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-353); mCherry: Met1 Deleted

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAP14

   Plasmid

   #186262

   Purpose

   wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Inserts

   wgaAB bicistronic
   EL222 (ELI_04755 Erythrobacter litoralis)
   

   Expression

   Bacterial

   Promoter

   LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-2T-TRR-C421-G2304D

   Plasmid

   #182845

   Purpose

   mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRR 2011-2431 (trr Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   mutation G2304D (GGC/GAC)

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-2T-TRX-C751-E3616S

   Plasmid

   #182844

   Purpose

   mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRX (w Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX4T3-GST-NQO1(Y128F)

   Plasmid

   #177141

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   NQO1 (NQO1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.2-coHPDL H163A H258A 3xFLAG-V5

   Plasmid

   #174131

   Purpose

   Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Codon optimized, H163A, H258A

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6.2-coHPDL H258A 3xFlag-V5

   Plasmid

   #174130

   Purpose

   Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Codon optimized, H258A

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-DEST42-dre4

   Plasmid

   #170440

   Purpose

   Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

   Depositor

   Joan Conaway

   Article

   Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

   Insert

   dre4 (dre4 Fly)
   

   Tags

   6xHis and V5

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-20

   Promoter

   T7

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMHE-mClover3-bElys

   Plasmid

   #171494

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged bovine Elys mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   Elys (AHCTF1 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   mClover3

   Promoter

   T7

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX030

    Plasmid

    #167154

    Purpose

    MoClo-compatible Level 1 (position 5) vector encoding Neonothopanus nambi caffeoylpiruvate hydrolase nnCPH under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnCPH-ocsT
    

    Expression

    Plant

    Promoter

    p35s

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAB120

    Plasmid

    #167150

    Purpose

    MoClo-compatible Level 1 (position 1) vector encoding Kanamycin resistance cassette, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    pNOS-nptII-ocsT
    

    Expression

    Plant

    Promoter

    pNOS

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX022

    Plasmid

    #167148

    Purpose

    MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    C-part of fungal hispidin synthase, nnHispS
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX021

    Plasmid

    #167147

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    N-part of fungal hispidin synthase, nnHispS
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

    Plasmid

    #91806

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Residues A1529-G1534 replaced with a PreScission …

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

    Plasmid

    #91807

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    PreScission Protease site (LEVLFQGP) inserted bet…

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

    Plasmid

    #91818

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 1355 and lysine 1435 to alanines; …

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1-Flag-Rheb-12Rs

    Plasmid

    #165023

    Purpose

    expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb-12Rs (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    All the lysine residues except K19 and K120 are c…

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p1A Prk-

    Plasmid

    #162694

    Purpose

    p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Prk inactive (K20M S21A native protein, this map …

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330_gRNA_H19-DMR-2

    Plasmid

    #159930

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330_gRNA_H19-DMR-1

    Plasmid

    #159929

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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