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tet-pLKO shDUSP19 puro

Plasmid

#87796

Purpose

Expresses an inducible short hairpin targeting human DUSP19 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shDUSP19 (DUSP19 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta-N-terminus

Plasmid

#64677

Purpose

expresses only N terminal o WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

Only N terminus amino acids 1-160

Promoter

CMV

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-C-terminus

Plasmid

#64679

Purpose

expresses only C terminus of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

only C terminus amino acids 500-548

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Flag-Tipin-L195A

Plasmid

#23120

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag- and His

Expression

Insect

Mutation

Changed Leucine 195 to Alanine

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pAG227 CORO1C 3'UTR mut

Plasmid

#12030

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

CORO1C 3'UTR mut (CORO1C Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG83 LRRK1 3'UTR mut

Plasmid

#12058

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N4 3'UTR mut (LRRK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pDGB3omega2_[EBSn:mini35S:3x SV40-NLS:GUS:35Sterm]-[tNOS:BASTA:pNOS]

Plasmid

#254068

Purpose

GUS expression driven by EBSn

Depositor

Jose Alonso, Anna Stepanova

Article

Fernandez-Moreno et al Plant Biotechnol J. 2026 Feb;24(2):698-7

Insert

EBSn:mini35S:3x SV40-NLS:GUS:35Sterm

Use

Synthetic Biology

Expression

Plant

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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pDGB3omega1_[mEBSn:mini35S:3x SV40-NLS:GUS:35Sterm]-[tNOS:BASTA:pNOS]

Plasmid

#254070

Purpose

GUS expression driven by mEBSn

Depositor

Jose Alonso, Anna Stepanova

Article

Fernandez-Moreno et al Plant Biotechnol J. 2026 Feb;24(2):698-7

Insert

mEBSn:mini35S:3x SV40-NLS:GUS:35Sterm

Use

Synthetic Biology

Expression

Plant

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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pDGB3omega2_[EBSn-mini35S:3x SV40-NLS:GUS:term35S]-[pNOS:KANAMYCIN:tNOS]

Plasmid

#254072

Purpose

GUS expression driven by EBSn

Depositor

Jose Alonso, Anna Stepanova

Article

Fernandez-Moreno et al Plant Biotechnol J. 2026 Feb;24(2):698-7

Insert

EBSn:mini35S:3x SV40-NLS:GUS:35Sterm

Use

Synthetic Biology

Expression

Plant

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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pPD864 pLP Rosa26 EF1a attP EYFP-P2A-Hygro

Plasmid

#253872

Purpose

Contains the landing pad (attP) flanked by Rosa26 homology arms to generate B16F10-LP

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

attP EYFP-P2A-Hygro

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1359 HBS(YBTATA) LumiScarlet in TU4

Plasmid

#253911

Purpose

Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and LumiScarlet in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

HBS(YB_TATA) LumiScarlet

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Promoter

HBS(YB_TATA)

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1358 HBS(YBTATA) LumiScarlet in TU3

Plasmid

#253910

Purpose

Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and LumiScarlet in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

HBS(YB_TATA) LumiScarlet

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Promoter

HBS(YB_TATA)

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1357 HBS(YBTATA) LumiScarlet in TU2

Plasmid

#253909

Purpose

Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and LumiScarlet in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

HBS(YB_TATA) LumiScarlet

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Promoter

HBS(YB_TATA)

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1402 Dox-Inducible mNeonGreen

Plasmid

#253891

Purpose

Integration Vector for mNeonGreen with the TRE3GV promoter (TUPV1), TetOn3G with the CAG promoter (TUPV2), and BlastR with the CAG promoter (TUPV3)

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mNeonGreen, TetOn3G, and BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GV, CAG, CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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AraC-S170-LOV-C450A-K488N(M393)

Plasmid

#250777

Purpose

Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

AraC with an AsLOV2 insertion behind S170 and C450A and K488N mutation

Use

Synthetic Biology

Tags

His-tag

Expression

Bacterial

Mutation

C450A, K488N

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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AraC-S170-LOV-C450A-D432V(M392)

Plasmid

#250776

Purpose

Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

AraC with an AsLOV2 insertion behind S170 and C450A and D432V mutation

Use

Synthetic Biology

Tags

His-tag

Expression

Bacterial

Mutation

C450A, D432V

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A-3A)

Plasmid

#218776

Purpose

Expresses dCAS9-DNMT3A-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A-3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A-KRAB (dCas9-3A-KRAB)

Plasmid

#218781

Purpose

Expresses dCAS9-DNMT3A-KRAB fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A-DNMT3L (dCas9-3A-3L)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-dDNMT3A (dCas9-d3A)

Plasmid

#218787

Purpose

Expresses dCAS9-dead DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-dDNMT3A (dCas9-d3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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bRA66-Mic60chimeras

Plasmid

#245525

Purpose

Edited bRA66 containing gRNA to create S. cerevisiae Mic60 chimeras

Depositor

Luke Chao

Article

Benning et al Protein Sci. 2025 Jul;34(7):e70207. doi:

Insert

YKR016w (MIC60 Budding Yeast)

Use

CRISPR

Promoter

T7 TAATACGACTCACTATAGG

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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