We narrowed to 10,337 results for: plasmids 101

1. pBABR IRFP-Bcd-LEXY

   Plasmid

   #182595

   Purpose

   Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bcd (bcd Fly)
   

   Use

   Tags

   IRFP and LEXY

   Expression

   Insect

   Mutation

   Promoter

   maternal tubulin

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH

   Plasmid

   #74289

   Purpose

   pAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   H2B-GFP and oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   Ef1a

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. (364) SB KRAB-SadCas9

   Plasmid

   #163022

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TET-On

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 Flag Fli1

   Plasmid

   #66976

   Purpose

   Expression plasmid for mouse Fli1; adds Flag tag to the N-terminus of protein

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Fli1 (Fli1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5/FRT/TO-Intron-CterFlag-MePCE

   Plasmid

   #113549

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. B270 + SPRTN sgSTOP

   Plasmid

   #100718

   Purpose

   B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SPRTN (cloned using BbsI)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

   Plasmid

   #99144

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xFlag-HaloTag-EZH2 HDR - pDY053

   Plasmid

   #171108

   Purpose

   Homology directed repair plasmid for generating HaloTag-EZH2 cells

   Depositor

   Thomas Cech

   Article

   Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9-beta-mmr

   Plasmid

   #169241

   Purpose

   Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   mutL-E36K
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed glutamic acid 36 to lysine

   Promoter

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

    Plasmid

    #102986

    Purpose

    AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    membrane-targeted SNAP protein after FLP-mediated recombination
    Nuclear-targeted eGFP after FLP-mediated recombination
    

    Use

    AAV; Adeno associated viral vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-GFP-EBA175-Flag

    Plasmid

    #154970

    Purpose

    Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    GFP and signal peptide (residues 1 to 32) from TG…

    Expression

    Mammalian

    Mutation

    insert codes for EBA175 residues 1284 to 1462

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

    Plasmid

    #163698

    Purpose

    AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

    Depositor

    Gina Turrigiano

    Article

    Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

    Insert

    CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII fragment

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV:: HA-hM4Dnrxn

    Plasmid

    #52525

    Purpose

    Expresses N-terminal HA tagged hM4Dnrxn

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    HA-hM4Dnrxn (CHRM4 Synthetic, Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-EF1a-AcGFP-P2A-hBRD4S

    Plasmid

    #197882

    Purpose

    study roles of human BET proteins in human pluripotency reprogramming

    Depositor

    Kejin Hu

    Article

    Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. YFP-Stim1-D76A

    Plasmid

    #186622

    Purpose

    Encodes Stim1 mutant D76A fused to YFP

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Stromal Interaction Molecule 1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    D76A

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-RORb2

    Plasmid

    #73999

    Purpose

    Overexpression of RORb2

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    RORb2 ORF (Rorb Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. (517) SB KRAB-SadCas9 U6-gStop

    Plasmid

    #163023

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAGGS-Flex-Turbo/GFP

    Plasmid

    #197886

    Purpose

    Can be used to generate AAV virus that will express Turbo/GFP in the presence of Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    Turbo/GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmKO-AF8

    Plasmid

    #118224

    Purpose

    Kusabira- Orange-CENP-A expression plasmid

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Mutat Res. 2008 Nov 17;657(1):56-62. doi

    Insert

    CENP-A (CENPA Human)
    

    Use

    Tags

    Kusabira orange

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN3-sTRAIL

    Plasmid

    #154246

    Purpose

    Plasmid compatible with non-viral delivery for secretion of trimeric human TRAIL

    Depositor

    Jordan Green

    Article

    Vaughan et al Mol Ther Oncolytics. 2021 Apr 16;21:377-

    Insert

    Secretable Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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