We narrowed to 10,632 results for: t7

1. pET28b-bhaB1

   Plasmid

   #167811

   Purpose

   Expresses His6-tagged BhaB1 in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

   Insert

   BhaB1
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-bhaB7

   Plasmid

   #167813

   Purpose

   Expresses His6-tagged BhaB7 in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

   Insert

   BhaB7
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b-bhaC1

   Plasmid

   #167814

   Purpose

   Expresses BhaC1 in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

   Insert

   BhaC1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBlueScript II SK-SNAP donor for Hist1h3g

   Plasmid

   #186932

   Purpose

   Genomic targeting of SNAP tag at Hist1h3g C-terminal

   Depositor

   Zhiguo Zhang

   Article

   Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

   Insert

   SNAP donor for Hist1h3g (H3c8 Mouse)
   

   Tags

   SNAP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET3a-FruK

   Plasmid

   #186256

   Purpose

   Overexpression of FruK for purification

   Depositor

   Emile Van Schaftingen, Liskin Swint-Kruse

   Article

   Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

   Insert

   1-phosphofructokinase (fruK E. coli)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SNAP-Kindlin

   Plasmid

   #186136

   Purpose

   For expression of recombinant Snap-labeled kindlin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   FERMT2 (FERMT2 Human)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSF-1b-S7-S83C/Δ157-178

   Plasmid

   #128592

   Purpose

   Expresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   S7 E.coli ribosomal protein (rpsG E.coli)
   

   Expression

   Bacterial

   Mutation

   S83C, Δ157-178

   Promoter

   T7

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHBT-Mb(GDx-Clo_L10)

   Plasmid

   #183406

   Purpose

   Expresses Monobody GDx-Clo_L10 in E. coli

   Depositor

   Shohei Koide

   Article

   Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

   Insert

   His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
   

   Tags

   His tag-Avi tag-TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6xHis(TEV)-Drp1(K569A,K571A)-StrepII

   Plasmid

   #174426

   Purpose

   Expresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   K569A, K571A

   Promoter

   T7

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCm N-term MBP SMT3 NikJC199U

    Plasmid

    #174363

    Purpose

    Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Tags

    8xHis, MBP, SMT3, and TEV

    Expression

    Bacterial

    Mutation

    C199U

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sc NybbR eRF3∆165N (H348E)

    Plasmid

    #175420

    Purpose

    Encodes His-MBP-TEV-ybbR-H348E eRF3∆165N

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF3 (SUP35 Budding Yeast)
    

    Tags

    His-MBP-TEV and ybbR

    Expression

    Bacterial

    Mutation

    ∆165N, H348E

    Promoter

    T7

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175788

    Purpose

    Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    AviTag, Hexahistidine tag, and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xHis(TEV)-Drp1(K557A,K560A)-StrepII

    Plasmid

    #174425

    Purpose

    Expresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K557A, K560A

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-kcnj12a

    Plasmid

    #173141

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj12a (kcnj12a Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-D-kcnj16

    Plasmid

    #173146

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj16a (kcnj16 Zebrafish)
    

    Use

    Pcr cloning vector

    Mutation

    Stop codon removed

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-11a HIV-1 P6

    Plasmid

    #154201

    Purpose

    Bacterial expression of Gag P6 from HIV-1 HXB2 strain

    Depositor

    Lalit Deshmukh

    Article

    Ramaraju et al Biochemistry. 2021 Aug 3. doi: 10.1021/a

    Insert

    Gag P6 protein from HIV-1 clone HXB2
    

    Tags

    GB1-6xHis Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ash1

    Plasmid

    #169711

    Purpose

    Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)

    Depositor

    Tanja Mittag

    Article

    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

    Insert

    Ash1 (ASH1 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Truncation including encoding amino acids 420-500

    Promoter

    T7

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET11a-Z-N-wtBlc

    Plasmid

    #168258

    Purpose

    N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

    Insert

    N-fragment of wtBlc + Leucine Zipper
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET19b-rDAD2 cPGFP(N180)

    Plasmid

    #166001

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(N180)
    

    Expression

    Bacterial

    Mutation

    LSSmOrange- V49A (please see depositor comments)

    Promoter

    T7 promoter

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. OfCasp3b

    Plasmid

    #166054

    Purpose

    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

    Depositor

    Clay Clark

    Article

    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

    Insert

    Orbicella faveolata caspase-3b
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7 lac promoter

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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