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AAV-CAG-GFP

Plasmid

#243759

Purpose

AAV plasmid expressing GFP under the CAG promoter.

Depositor

Andrew Arrant

Article

Nader et al Sci Rep. 2025 Jul 19;15(1):26217. doi: 1

Insert

Enhanced Green Fluorescent Protein

Use

AAV

Promoter

CAG

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_2-3_005_Promoter+5'UTR_Placeholder

Plasmid

#235870

Purpose

Encodes a sfGFP cassette in the promoter+5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Promoter + 5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244076

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.H348H.HaloTag

Plasmid

#244108

Purpose

Bacterial expression of green glucose sensor (high affinity) with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Tags

HaloTag

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010C-Eco1

Plasmid

#240690

Purpose

RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by J23115 constitutive promoter

Depositor

Seth Shipman

Article

González-Delgado et al bioRxiv 2025.06.16.660010

Insert

Eco1 RT, Eco1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pYO843

Plasmid

#242487

Purpose

Expression of ScAtg38 Δ(210 to 226)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 210 to 226

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO835

Plasmid

#242484

Purpose

Expression of ScAtg38 Δ(123 to 181)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 123 to 181

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO842

Plasmid

#242486

Purpose

Expression of ScAtg38 Δ(123 to 209)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 123 to 209

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO817

Plasmid

#242483

Purpose

Expression of ScAtg38 Δ(182 to 226)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 123 to 226

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO836

Plasmid

#242485

Purpose

Expression of ScAtg38 Δ(182 to 209)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 182 to 209

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010-opEbu1

Plasmid

#240702

Purpose

RSF1010 origin of replication plasmid containing Ebu1 recombitron with extended a1 a2 regions with a donor in the ncRNA targeting lacZ locus in Citrobacter freundii ATCC 8090 expressed by Pm promoter

Depositor

Seth Shipman

Article

González-Delgado et al bioRxiv 2025.06.16.660010

Insert

Ebu1 RT, Ebu1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

Extended a1 a2 regions

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010-Abe1

Plasmid

#240683

Purpose

RSF1010 origin of replication plasmid containing Abe1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Article

González-Delgado et al bioRxiv 2025.06.16.660010

Insert

Abe1 RT, Abe1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_D11 (pAVA3773)

Plasmid

#239310

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and a non-targeting sgRNA as control(-)

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven non-targeting sgRNA Control(-)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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p-HITI-GFP-Ckm

Plasmid

#226119

Purpose

HITI insert construct with Ckm gRNA and GFP transgene

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Insert

Ckm (Ckm Mouse)

Use

CRISPR

Tags

eGFP

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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TTR A25T

Plasmid

#238114

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

A25T

Promoter

T7

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR A25T)

Plasmid

#238116

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

A25T

Promoter

T7

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-IE1

Plasmid

#235541

Purpose

To express tagBFP-2A-IE1

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

IE1

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-FLAG-coORF63

Plasmid

#235544

Purpose

To express tagBFP-2A-FLAG-coORF8 (co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ORF63

Use

Lentiviral

Tags

FLAG

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo-MORC3_gRNA_1

Plasmid

#235529

Purpose

gRNA against human MORC3

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

MORC3 (MORC3 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo-MORC3_gRNA_3

Plasmid

#235530

Purpose

gRNA against human MORC3

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

MORC3 (MORC3 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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