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PE-SUMO CRYAB

Plasmid

#217670

Purpose

For expression of SUMO tagged human alpha B crystallin

Depositor

Larry David

Insert

CRYAB (CRYAB Human)

Tags

6XHis and Smt3

Expression

Bacterial

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_mCherry_2A_HA_2xMS2-VP64

Plasmid

#68419

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

MS2

Use

Synthetic Biology

Tags

HA and VP64

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

CMV

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGEM cCol2 (CT#617)

Plasmid

#13943

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

Collagen II (COL2A1 Chicken)

Expression

Bacterial

Mutation

Nucleotides 182-616

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBS-67Sma

Plasmid

#49342

Purpose

Chlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic region

Depositor

Anastasios Melis

Article

Chen et al Plant Biotechnol J. 2013 Sep;11(7):818-2

Insert

EcoRI 5.8 kB

Use

Synthetic Biology

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pGEM cAgg (CT#689)

Plasmid

#13945

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

Aggrecan (ACAN Chicken)

Expression

Bacterial

Mutation

Nucleotides 113-800

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRKO

Plasmid

#49456

Purpose

loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

Depositor

Marc Gartenberg

Article

Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

Insert

loxP-kanMX-loxP cassette

Use

Cre/Lox

Expression

Bacterial

Promoter

none

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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ciCas9_pcDNA5

Plasmid

#100550

Purpose

Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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CDC42

Plasmid

#55693

Purpose

expression of mouse CDC42

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

CDC42

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pFA-MNase-SAT1

Plasmid

#162465

Purpose

pFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNase

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase

Expression

Yeast

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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130_pSKB3-LUMP-P.leioghnati

Plasmid

#65894

Purpose

Bacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetime

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Expression

Bacterial

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pB_35S/mEGFP

Plasmid

#135320

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-ConVERGD-eGFP-WPRE

Plasmid

#218747

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

nEF

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-ConVERGD-eGFP-W3SL

Plasmid

#218746

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

nEF

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Cdh1

Plasmid

#49776

Purpose

pENTR vector for mouse E-cadherin

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Cdh1 (Cdh1 Mouse)

Use

Entry vector

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
REM2

Plasmid

#55682

Purpose

expression of mouse REM2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

REM2 (Rem2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
mGarnet2-RITA

Plasmid

#104314

Purpose

codes for a fusion of fluorescent protein mGarnet2 and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

mGarnet2-RITA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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RASD1

Plasmid

#55671

Purpose

expression of mouse RASD1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASD1 (Rasd1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pKDH

Plasmid

#67584

Purpose

Gene expression in plants. A promoter and/or reporter can be cloned into this plasmid

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Type

Empty backbone

Expression

Plant

Promoter

None

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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RIT1

Plasmid

#55662

Purpose

expression of mouse RIT1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RIT1 (Rit1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti_9xD3B_Cluc_Norm

Plasmid

#68414

Purpose

CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Cypridina Luciferase-2A-Venus YFP

Use

Lentiviral and Luciferase

Mutation

YFP: M153T,V163A,S175G ("Venus") YFP Va…

Promoter

minimalCMV

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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