We narrowed to 11,278 results for: Vars;

1. pcDNA3.1-Gα13(EE)-YFP

   Plasmid

   #171374

   Purpose

   Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Ga13(EE)-YFP
   

   Tags

   Glu-Glu

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAc5.1B-EGFP-DmDCP2-V5His6

   Plasmid

   #22410

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

   Insert

   decapping protein 2, variant A (DCP2 Fly)
   

   Use

   Drosophila expression

   Tags

   EGFP and V5-His6

   Available Since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAZ10::LUC

   Plasmid

   #141172

   Purpose

   Luminescent reporter for jasmonic acid signaling

   Depositor

   Patrick Schäfer, Vardis Ntoukakis

   Article

   Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

   Insert

   AtJAZ10 promoter
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   AtJAZ10, AT5G13220

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330 EQR

   Plasmid

   #101733

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-EGFP-DmXRN1_A

   Plasmid

   #145874

   Purpose

   Insect Expression of DmXRN1

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmXRN1 (pcm Fly)
   

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET11a-DAAT-V33G/T242G

   Plasmid

   #159278

   Purpose

   Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

   Depositor

   Roberto Chica

   Article

   Parmeggiani et al ACS Catalysis 9, 3482–3486.

   Insert

   Engineered d-alanine aminotransferase
   

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Valine 33 and threonine 242 have been mutated to …

   Promoter

   T7

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. P315-FBP_10

   Plasmid

   #139701

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and Npga

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF(UG) hSyn SYP-SELF

   Plasmid

   #158770

   Purpose

   Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   Synaptophysin 1-NS3/4a (Syt1 Mouse)
   

   Use

   Lentiviral

   Mutation

   Flexible linkers, ADVVCC/QMSY cleavage site

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWRKY70::LUC

   Plasmid

   #141171

   Purpose

   Luminescent reporter for salicylic acid signaling

   Depositor

   Patrick Schäfer, Vardis Ntoukakis

   Article

   Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

   Insert

   AtWRKY70 promoter
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   AtWRKY70, AT3G56400

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)

    Plasmid

    #126026

    Purpose

    express RFP-tagged LARIAT variant for optogenetic clustering

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CIBN-MP; CRY2 PHRolig-VHH(GFP)
    

    Tags

    RFP

    Expression

    Insect

    Mutation

    CRY2 E490G

    Promoter

    Hsp70

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-his-3C_Clu3B6p

    Plasmid

    #100093

    Purpose

    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

    Depositor

    Nikolai Sluchanko

    Article

    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

    Insert

    14-3-3 sigma human (SFN Human)
    

    Tags

    HSPB6 peptide and His6

    Expression

    Bacterial

    Mutation

    75-77 residues changed to AAA to facilitate cryst…

    Promoter

    T7

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. UAS::PTPRDb-DN-GFP

    Plasmid

    #37112

    Depositor

    Alvaro Sagasti

    Article

    Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

    Insert

    UAS::PTPRDb-DN-GFP (ptprdb Zebrafish)
    

    Use

    Tol2 gateway destination vector

    Tags

    EGFP

    Mutation

    deleted cytodomain, including phosphatase domains…

    Promoter

    UAS

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. SEPT11 sh2 shRNA

    Plasmid

    #122023

    Purpose

    Knock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 shRNA (Sept11 Rat)
    

    Use

    RNAi

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYC25

    Plasmid

    #63914

    Purpose

    Recipient overexpressing vector to construct YFP tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Tags

    YFP

    Expression

    Yeast

    Promoter

    TEF1 (Saccharomyces cerevisiae)

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1B-lambdaN-HA-DmDCP2-V5His6

    Plasmid

    #22409

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

    Insert

    decapping protein 2, variant A (DCP2 Fly)
    

    Use

    Drosophila expression

    Tags

    V5-His6 and lambdaN-HA

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. EcPhnD90.L1∆∆AD.L297RL301R.pRSET

    Plasmid

    #33376

    Purpose

    a single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFP

    Depositor

    Loren Looger

    Article

    Alicea et al J Mol Biol. 2011 Oct 12.

    Insert

    phnD based Phosphonate Biosensor (phnD E. coli)
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted in place of…

    Promoter

    T7

    Available Since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYC16

    Plasmid

    #63913

    Purpose

    Recipient overexpressing vector to construct CFP tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Tags

    CFP

    Expression

    Yeast

    Promoter

    TEF1 (Saccharomyces cerevisiae)

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF-HA/Net1 Δ1-121

    Plasmid

    #28263

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1 (Net1 Mouse)
    

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Missing aa 1-121

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pAG mKappa Anti-c-Myc [9E10]

    Plasmid

    #242956

    Purpose

    Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse kappa light chain constant region; to be used with pAG mIgG2a Anti-c-Myc [9E10] heavy chain (Plasmid 242955) to make the antibody.

    Depositor

    Meghan Rego, Addgene Research Program

    Insert

    Anti-c-Myc [9E10] light chain variable region fused to mouse kappa light chain constant region
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. tetO-HyperTRIBE-adar_deaminase-V5-t2a-mCherry

    Plasmid

    #241314

    Purpose

    inducible mouse Adar Deaminase domain (HyperTRIBE) for expression in mammalian cells

    Depositor

    Stavros Lomvardas

    Insert

    Adar deaminase domain (Adar Mouse)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    E957Q

    Promoter

    tetO and CMV

    Available Since

    Oct. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. p305N

    Plasmid

    #246316

    Purpose

    A promoterless construct designed for cloning various Pol III promoters or serving as a control in evaluating Pol III promoters for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and poplar reporter lines

    Depositor

    Chung-Jui Tsai

    Article

    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

    Insert

    Promoterless
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Promoterless

    Available Since

    Oct. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. p305N-8

    Plasmid

    #246291

    Purpose

    Evaluation of AtU6.29c14 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

    Depositor

    Chung-Jui Tsai

    Article

    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

    Insert

    AtU6.29c14 promoter
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    T-to-C (-1 from TSS)

    Promoter

    AtU6.29c14 promoter

    Available Since

    Oct. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. p305N-6

    Plasmid

    #246289

    Purpose

    Evaluation of AtU6.1m3 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

    Depositor

    Chung-Jui Tsai

    Article

    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

    Insert

    AtU6.1m3 promoter
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    G-to-T (-57 from TSS) within USE

    Promoter

    AtU6.1m3 promoter

    Available Since

    Oct. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. p305N-28

    Plasmid

    #246311

    Purpose

    Evaluation of PtU6.1c1 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

    Depositor

    Chung-Jui Tsai

    Article

    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

    Insert

    PtU6.1c1 promoter
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    C-to-A (-42 from TSS), A-to-C (-41)

    Promoter

    PtU6.1c1 promoter

    Available Since

    Oct. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. p305N-14

    Plasmid

    #246297

    Purpose

    Evaluation of CiU6.6c16 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

    Depositor

    Chung-Jui Tsai

    Article

    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

    Insert

    CiU6.6c16 promoter
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    T-to-C (-2 from TSS)

    Promoter

    CiU6.6c16 promoter

    Available Since

    Oct. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p305N-11

    Plasmid

    #246294

    Purpose

    Evaluation of CiU6.3c8 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

    Depositor

    Chung-Jui Tsai

    Article

    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

    Insert

    CiU6.3c8 promoter
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    A-to-C (-23 from TSS)

    Promoter

    CiU6.3c8 promoter

    Available Since

    Oct. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p305N-16

    Plasmid

    #246299

    Purpose

    Evaluation of HbU6.2m1 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

    Depositor

    Chung-Jui Tsai

    Article

    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

    Insert

    HbU6.2m1 promoter
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    A-to-G (-56 from TSS), C-to-T (-29), G-to-A (-24)

    Promoter

    HbU6.2m1 promoter

    Available Since

    Oct. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSYL017

    Plasmid

    #246593

    Purpose

    template plasmid for site-saturation mutagenesis library cloning

    Depositor

    Tina Wang

    Article

    Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

    Insert

    AtRaf1 variant 2g (AT3G04550 Mustard Weed)
    

    Expression

    Bacterial

    Mutation

    AtRaf1L121F/I127L/A195V/N351Y/R432G

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. p15GFPkv

    Plasmid

    #239039

    Purpose

    Expresses a + positively charged GFP variant (+15GFPKv) in mammalian cells

    Depositor

    Felipe Quiroz

    Article

    Avecilla et al ACS Sens. 2025 Mar 28;10(3):1857-1869. d

    Insert

    p15GFPKv-NES
    

    Expression

    Mammalian

    Mutation

    All surface-exposed Arg residues changed to Lysine

    Promoter

    CMV

    Available Since

    Oct. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. IKKε v2-V5

    Plasmid

    #169770

    Purpose

    Express IKKε v2 in mammalian cells

    Depositor

    Sung-Liang Yu

    Article

    Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

    Insert

    Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2
    

    Tags

    V5 tag

    Expression

    Bacterial and Mammalian

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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