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pT7-EGFP-C1-HsR3HCC1_39-480_K

Plasmid

#146764

Purpose

Mammalian Expression of HsR3HCC1_39-480

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsR3HCC1_39-480 (R3HCC1 Human)

Expression

Mammalian

Mutation

sequence extracted from BC050572.1; 39-480 trunca…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTDRD3_K

Plasmid

#146768

Purpose

Mammalian Expression of HsTDRD3

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsTDRD3 (TDRD3 Human)

Expression

Mammalian

Mutation

two non silent mutations H100R and P508H compared…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTDRD3-EBMmut_K

Plasmid

#146769

Purpose

Mammalian Expression of HsTDRD3-EBMmut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsTDRD3-EBMmut (TDRD3 Human)

Expression

Mammalian

Mutation

two non silent mutations H100R and P508H compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-515_I

Plasmid

#146566

Purpose

Bacterial Expression of HsSmg6_1-515

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-515 (SMG6 Human)

Expression

Bacterial

Mutation

two silent mutations and two non silent mutation …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-3-GST-DmGW182_1-593_F

Plasmid

#146271

Purpose

Bacterial Expression of DmGW182_1-593

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmGW182_1-593 (gw Fly)

Expression

Bacterial

Mutation

one non silent mutation T1772A (L591Q) compared t…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_578-634-GB1_AG

Plasmid

#148744

Purpose

Bacterial Expression of DmeIF4G_578-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_578-634 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-AAGAB

Plasmid

#198332

Purpose

Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AAGAB (AAGAB Human)

Tags

GAL4-DNA binding domain (BD) fragment

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-DmCaf40_25-291-Y139DG146W_AF

Plasmid

#148736

Purpose

Bacterial Expression of DmCaf40_25-291-Y139DG146W

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40_25-291-Y139DG146W (Rcd-1 Fly)

Expression

Bacterial

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_377-428_AG

Plasmid

#148757

Purpose

Mammalian Expression of HsNot4_377-428

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_377-428 (CNOT4 Human)

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TERTp124_Gluc_CCR5

Plasmid

#192271

Purpose

Donor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Mutation

-124 C to T

Promoter

TERT promoter driving expression of gaussia

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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TERTp146_Gluc_CCR5

Plasmid

#192272

Purpose

Donor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Mutation

-146 C to T

Promoter

TERT promoter driving expression of gaussia

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Sa-gRNA-puro

Plasmid

#191652

Purpose

Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

S. aureus gRNAscr

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-HsGIGYF1_1-671_AI

Plasmid

#148958

Purpose

Mammalian Expression of HsGIGYF1_1-671

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1_1-671 (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1_1-671_AI

Plasmid

#148943

Purpose

Mammalian Expression of HsGIGYF1_1-671

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1_1-671 (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1-F306AF312A_AI

Plasmid

#148945

Purpose

Mammalian Expression of HsGIGYF1-F306AF312A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1-F306AF312A (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1-W294A_AI

Plasmid

#148946

Purpose

Mammalian Expression of HsGIGYF1-W294A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1-W294A (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1-W294AF306AF312A_AI

Plasmid

#148947

Purpose

Mammalian Expression of HsGIGYF1-W294AF306AF312A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1-W294AF306AF312A (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-MBP-GGSG-DmBam_13-36_AG

Plasmid

#148841

Purpose

Mammalian Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-DmRoquin-del725-755-del790-812_AE

Plasmid

#148634

Purpose

Mammalian Expression of DmRoquin-del725-755-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin-del725-755-del790-812 (roq Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0428 huDisCas7-11-R1045-GGGS-R1122-U6-pro-Gluc

Plasmid

#186985

Purpose

AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of Gluc crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122, Gluc crRNA guide

Use

AAV

Mutation

r1045-gggs-r1122 deletion

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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