We narrowed to 17,703 results for: puromycin

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RPS6KA1 gRNA (BRDN0001145974)

Plasmid

#75497

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA1 (RPS6KA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EIF2AK3 gRNA (BRDN0001146328)

Plasmid

#77167

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK3 (EIF2AK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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MO70-N-Flag-hCRACR2A-isoform-a

Plasmid

#79592

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Publication

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
ERN1 gRNA (BRDN0001162304)

Plasmid

#76407

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERN1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERN1 (ERN1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SGK3 gRNA (BRDN0001146281)

Plasmid

#75541

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C8orf44-SGK3,SGK3 (SGK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO_UCA1

Plasmid

#72628

Purpose

Expresses two gRNAs targeting the UCA1 promoter

Depositor

Roderic Guigo, Rory Johnson

Publication

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward UCA1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO_Malat1_B

Plasmid

#72621

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Roderic Guigo, Rory Johnson

Publication

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNAs toward Malat1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV_mIFP IRES EGFP

Plasmid

#72443

Purpose

Expressing mIFP and EGFP in mammalian cells

Depositor

Xiaokun Shu

Publication

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

mIFP T2A HO1 IRES EGFP

Use

Lentiviral

Expression

Mammalian

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

Plasmid

#75118

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

Depositor

Christopher Vakoc

Publication

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRD7

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pLCKO_EIF3D_sgRNA_4

Plasmid

#74188

Purpose

lentiviral vector expressing sgRNA targeting EIF3D

Depositor

Jason Moffat

Publication

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

EIF3D sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLCKO_EIF3D_sgRNA_1

Plasmid

#74187

Purpose

lentiviral vector expressing sgRNA targeting EIF3D

Depositor

Jason Moffat

Publication

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

EIF3D sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMB2_sgRNA_1

Plasmid

#74184

Purpose

lentiviral vector expressing sgRNA targeting PSMB2

Depositor

Jason Moffat

Publication

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMB2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HuEV-A

Plasmid

#68342

Purpose

Gateway compatible human expression vector that adds a "flexible" 3XFLAG,V5,YFP N terminus tag

Depositor

Jef Boeke

Publication

Mita et al Biol Proced Online. 2016 Aug 15;18:16. d

Type

Empty backbone

Use

Cre/Lox

Tags

3xFLAG, V5 and YFP

Expression

Mammalian

Promoter

TET-CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXs-Puro-CLEC16A-EMP2

Plasmid

#69468

Purpose

Expresses CLEC16A-EMP2 fusion gene

Depositor

Axel Hillmer

Publication

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

CLEC16A-EMP2 (CLEC16A, EMP2 Human)

Use

Retroviral

Tags

HA TAG

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EGFP-C3-Cep123

Plasmid

#73330

Purpose

Lentiviral vector encoding Cep123 isoform 1 with an N-terminal EGFP tag

Depositor

Manuel Thery

Insert

Cep123 (CEP89 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-ONL-CP

Plasmid

#65716

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST

Use

Luciferase

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-ONL

Plasmid

#65713

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

7xTcf, minCMV, 3xNLS, ONL

Use

Luciferase

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Rgl2-CAAX-pcw107-V5

Plasmid

#64641

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Rgl2 plus C-term KRAS (Kras Mouse)

Use

Lentiviral

Tags

V5

Mutation

C-term of Kras

Promoter

PGK

Available since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only
myr-FLAG-MEK5-pcw107

Plasmid

#64620

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Lentiviral

Tags

Flag and myristoylated

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
JNK2 WT O/E (MAPK9)-pcw107-V5

Plasmid

#64617

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAPK9 (transcript variant JNK2-a2) (MAPK9 Human)

Use

Lentiviral

Tags

V5

Mutation

none

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only