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pGL4_10xUAS-CMVmin-BC0404-luc2

Plasmid

#214631

Purpose

barcoded assay, UAS sensor for split TEV assays

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

10x clustered UAS element with barcode 0404

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0389-luc2

Plasmid

#214625

Purpose

barcoded assay, UAS sensor for split TEV assays

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

10x clustered UAS element with barcode 0389

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0388-luc2

Plasmid

#214624

Purpose

barcoded assay, UAS sensor for split TEV assays

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

10x clustered UAS element with barcode 0388

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0401-luc2

Plasmid

#214628

Purpose

barcoded assay, UAS sensor for split TEV assays

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

10x clustered UAS element with barcode 0401

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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β2AR-PPI-CALPon1.0 (TEVp)

Plasmid

#213407

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

HA-arrestin-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS106

Plasmid

#209359

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal moxMaple fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS99

Plasmid

#209327

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of untagged protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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H2-pAc5.1 mCherry Oskar 139-606

Plasmid

#206491

Purpose

Oskar 139-606 expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-606

Tags

mCherry

Expression

Insect

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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F16-pAc5.1 EGFP Vasa closed

Plasmid

#206487

Purpose

Vasa (closed conformation) expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

vasa E400Q

Tags

EGFP

Expression

Insect

Mutation

E400Q

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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F15-pAc5.1 EGFP Vasa

Plasmid

#206483

Purpose

Vasa expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Vasa

Tags

EGFP

Expression

Insect

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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F20-pAc5.1 EGFP Aubergine

Plasmid

#206482

Purpose

Aubergine expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Aubergine

Tags

EGFP

Expression

Insect

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMXSIP-TMEM141-FLAG

Plasmid

#204702

Purpose

expresses TMEM141-FLAG in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

TMEM141 (TMEM141 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2403

Plasmid

#195642

Purpose

Complementation of MlaC(WT)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Article

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0120

Plasmid

#172791

Purpose

Level 0 module containing CDS2ns_mHSP21

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature HSP21 (44-227), Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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LDB221

Plasmid

#185427

Purpose

Bacterial expression of Nup1 C-terminal residues 777-1076 as GST fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal residues 777-1076

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB203

Plasmid

#185424

Purpose

Bacterial expression of N-terminus of NUP1 nucleoporin as GST-Nup1-N fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 N-terminal domain only

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB205

Plasmid

#185425

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal domain only

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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KBB101

Plasmid

#185064

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after the last FXFG repeat

Depositor

Ken Belanger

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal region, truncated

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT-AIDm-EGFP

Plasmid

#182044

Purpose

in vitro transcription of AIDm-EGFP

Depositor

Janice Evans

Article

Camlin et al Biol Reprod. 2019 Oct 25;101(4):704-718.

Insert

AIDm-EGFP

Use

In vitro transcription

Tags

EGFP

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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