We narrowed to 16,131 results for: form

1. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

   Plasmid

   #175784

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Tags

   AviTag, Hexahistidine tag, and eGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. C. difficile 16S toehold switch sensor

   Plasmid

   #110704

   Purpose

   Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   C. difficile 16S toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. JARID2-KO-gRNA-1

   Plasmid

   #131328

   Purpose

   gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   JARID2-KO-gRNA-1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

4. JARID2-KO-gRNA-2

   Plasmid

   #131327

   Purpose

   gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   JARID2-KO-gRNA-2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

5. pPAct2noUTR (GB0192)

   Plasmid

   #68177

   Purpose

   Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtAct2 promoter (no UTR)
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. ERAS

   Plasmid

   #55656

   Purpose

   expression of mouse ERAS

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   ERAS (Eras Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pET27b-ecDHFR-N23PP/S148A

   Plasmid

   #110829

   Purpose

   Bacterial expression of E.coli DHFR N23PP/S148A mutant

   Depositor

   Stephen Benkovic

   Article

   Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   N23 mutated to P23; a proline residue inserted be…

   Promoter

   T7

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. GEM

   Plasmid

   #55680

   Purpose

   expression of mouse GEM

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   GEM (Gem Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSECB

   Plasmid

   #106305

   Purpose

   Express spCas9 with sgRNA, blast resistance

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CRISPR V2 sgShmt2_2

    Plasmid

    #106315

    Purpose

    Express Cas9 and sgRNA targeting mShmt2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting mShmt2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF1-PTPRD-G1707R

    Plasmid

    #32526

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta G1707R (PTPRD Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G1707R

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

12. p3E-RhoAb

    Plasmid

    #109579

    Purpose

    Multisite gateway vector for 3' tagging with RhoAb

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    RhoAb (rhoab Zebrafish)
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbdCas9S(RBSmut)_Psyn-sgRNArodA

    Plasmid

    #149656

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tol2 Krt4 Src (YF)-uniRapR-cerulean

    Plasmid

    #45509

    Depositor

    Klaus Hahn, Anna Huttenlocher

    Article

    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    Src (YF)-uniRapR-cerulean
    

    Use

    Zebrafish expression

    Tags

    cerulean

    Mutation

    Y732F

    Promoter

    krt4

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCVpic2neo-EGFP-FF2

    Plasmid

    #164922

    Purpose

    Expresses EGFP cDNA and FF2 shRNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

    Inserts

    Enhanced green fluorescent protein
    Neuropeptide FF receptor 2 (NPFFR2 Human)
    

    Use

    Retroviral

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCASP-SptP120-NS1-HilA

    Plasmid

    #153327

    Purpose

    Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-NS1 Monobody
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pr2-deGFP-MGapt

    Plasmid

    #67736

    Purpose

    OR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T500
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr2

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pr1-deGFP-MGapt

    Plasmid

    #67735

    Purpose

    OR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T500
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr1

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. OccludinMut_Flag

    Plasmid

    #86043

    Purpose

    Full length Occludin with mutation of PPPY domain at AA9-12 to PAAY

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

    Insert

    OccludinMut (OCLN Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    PPPY domain at AA9-12 to PAAY; I52T and Q365P

    Promoter

    CMV

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCellFree_G03 CTNNB1

    Plasmid

    #67119

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only