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pGWB541

Plasmid

#74875

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-neo.ARRDC1

Plasmid

#38321

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAb-DN

Plasmid

#109580

Purpose

Multisite gateway vector for 3' tagging with dominant negative RhoAb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAb (rhoab Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBXNPHM3

Plasmid

#110099

Purpose

E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pR0306 CcaCas13b His6-TwinStrep-SUMO

Plasmid

#182687

Purpose

Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

CcaCas13b

Tags

SUMO

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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IRF3-V5

Plasmid

#169775

Purpose

Express IRF3 in mammalian cells

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1 (IRF3 Human)

Tags

V5 tag

Expression

Bacterial and Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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ChR2-2A-Halo

Plasmid

#22047

Purpose

Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

Depositor

Edward Boyden

Article

Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

Insert

ChR2-GFP-2A-Halo-YFP

Tags

GFP and YFP

Expression

Mammalian

Mutation

Note that there is a S147R mut in YFP. This mutat…

Promoter

CMV

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-CreiLOV-P2A-FusionRed

Plasmid

#192818

Purpose

Expresses CreiLOV with FusionRed tag in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

CreiLOV

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB435

Plasmid

#74825

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk3a2

Plasmid

#13759

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2 (MAPK10 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pTP288

Plasmid

#113460

Purpose

human Flag tagged Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Nbs1 (NBN Human)

Tags

Flag

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDelila (GB0079)

Plasmid

#68200

Purpose

Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Delila coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pRosea (GB0026)

Plasmid

#68194

Purpose

Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

A. majus Rosea coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-ChroME2f-mRuby2-ST

Plasmid

#170160

Purpose

Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2f-mRuby2-ST

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Cyclin D1

Plasmid

#19927

Depositor

Yue Xiong

Article

Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

Insert

Cyclin D1 (CCND1 Human)

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP

Plasmid

#111584

Purpose

An AAV vector that expresses myristoylated mScarlet-I and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrmScarlet-I-P2A-post-eGRASP

Use

AAV

Promoter

tetO

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB513

Plasmid

#74855

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a1

Plasmid

#13754

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a1 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pEK39

Plasmid

#210599

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter

Tags

mRuby3

Expression

Bacterial and Yeast

Promoter

SpH2Bpro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-FOXP2

Plasmid

#153146

Purpose

Expression of Flag tagged human FOXP2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXP2 (FOXP2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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