2,202 results
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Plasmid#227778PurposeDestination vector with U6 driven 4 sgRNAsDepositorInsert4xU6:sgRNA
UseCRISPRPromoterU6Available SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA
Plasmid#226834PurposeExpression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafishDepositorInsertsCas9-t2A-GFP
4xU6:sgRNA
UseCRISPRPromoterOlactb and U6Available SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol1 - 14xUAS:CaSR-EGFP
Plasmid#191502PurposeA UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.DepositorInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol1 transgenes…Promoter14X UASAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hcar1-4-mKate2
Plasmid#226712Purposeoverexpression red fluorescent hcar1-4 protein in zebrafish larvaeDepositorInserthcar1-4
UseUnspecified; Zebrafish expressionTagsmKate2PromoterSP6Available SinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-5.0dand5:EGFP
Plasmid#223327PurposeExpresses EGFP in Kupffer's vesicle cells in zebrafish embryosDepositorInsert5.0kb dand5 promoter (dand5 Zebrafish)
UseZebrafish expressionAvailable SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2D-A
Plasmid#221734PurposeExpression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2D-A (ago2 Zebrafish)
Tags3xFLAGExpressionMammalianMutationD683A in active sitePromoterCMVAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2DY-EF
Plasmid#221733PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
Tags3xFLAGExpressionMammalianMutationD651E and Y680F in active sitePromoterCMVAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2Y-F
Plasmid#221732PurposeExpression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2Y-F (ago2 Zebrafish)
Tags3xFLAGExpressionMammalianMutationY680F in active sitePromoterCMVAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2D-E
Plasmid#221731PurposeExpression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2D-E (ago2 Zebrafish)
Tags3xFLAGExpressionMammalianMutationD651E in active sitePromoterCMVAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2
Plasmid#221730PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.DepositorAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2Y-F
Plasmid#221715PurposeExpression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2Y-F (ago2 Zebrafish)
UseFish expressionTags3xFLAGMutationY680F in active sitePromotersimian CMV IE94Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2D-E
Plasmid#221714PurposeExpression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2D-E (ago2 Zebrafish)
UseFish expressionTags3xFLAGMutationD651E in active sitePromotersimian CMV IE94Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-drAgo2
Plasmid#221711PurposeExpression of zebrafish Ago2 in zebrafish embryos.DepositorInsertdrAgo2 (ago2 Zebrafish)
UseFish expressionAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-dr_Unc (JDW 1365)
Plasmid#224482PurposeGateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intronDepositorInsertUnc-503 promoter
UseGateway CloningAvailable SinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2
Plasmid#218208PurposeZebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_2
UseZebrafish expressionAvailable SinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2_-87prox1a:basEGFP_Acry:GFP
Plasmid#218213PurposeZebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lensDepositorInsert-87prox1a
UseZebrafish expressionAvailable SinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDR274-gsc2-sgRNA
Plasmid#184818PurposeUsed to synthesize gRNA targeting exon 2 of the gsc2 geneDepositorInsertgsc2-sgRNA
UseCRISPR; Template for sgrna synthesisAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP-rln3a-HA
Plasmid#184820PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.DepositorInsertrln3a-HA
UseCRISPR; For integration of qf2 by homology-direct…Available SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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GBH-2aG4-insBFP.1
Plasmid#188025PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker insulin BFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; insulin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-insGFP.1
Plasmid#188028PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker insulin GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; insulin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-insGFP.2
Plasmid#188029PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker insulin GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; insulin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-insBFP.2
Plasmid#188008PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker insulin BFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; insulin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-insBFP.3
Plasmid#188009PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker insulin BFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; insulin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-insGFP.1
Plasmid#188010PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker insulin GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; insulin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-insGFP.2
Plasmid#188011PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker insulin GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; insulin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-insGFP.3
Plasmid#188012PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker insulin GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; insulin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryBFP.1
Plasmid#188013PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; gamma crystallin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryBFP.2
Plasmid#188014PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; gamma crystallin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryGFP.1
Plasmid#188016PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; gamma crystallin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryGFP.2
Plasmid#188017PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; gamma crystallin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryGFP.3
Plasmid#188018PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; gamma crystallin promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2BFP.1
Plasmid#188019PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; cmlc2 promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2BFP.2
Plasmid#188020PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; cmlc2 promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2BFP.3
Plasmid#188021PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; cmlc2 promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2GFP.1
Plasmid#188022PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; cmlc2 promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2GFP.2
Plasmid#188023PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; cmlc2 promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2GFP.3
Plasmid#188024PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
UseTargetable insertional mutagen and reporter systemPromoterEndogenuous Promoter; cmlc2 promotorAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only