We narrowed to 4,492 results for: BRE

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EGFP-Hu_Plk5(W207F)

Plasmid

#136320

Purpose

Mammalian expression of human Plk5 W207F with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Expression

Mammalian

Mutation

W207F

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CAGGS-SAFB-NLS-3XFLAG-V5

Plasmid

#196089

Purpose

Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Promoter

CAGGS

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pB-rtTA

Plasmid

#126034

Purpose

PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.

Depositor

Mauro Calabrese

Article

Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

Insert

rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735

Use

Piggybac

Expression

Mammalian

Promoter

hUbiC

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast

Plasmid

#128010

Purpose

traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

Use

Drosophila osc reporter construct

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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HeLa full-length MLCK-GFP

Plasmid

#46316

Depositor

Anne Bresnick

Article

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase (MYLK Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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Hela kinase-dead MLCK-GFP

Plasmid

#46317

Depositor

Anne Bresnick

Article

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase-dead (MYLK Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

E1626K

Promoter

CMV

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only
pFBP_2xNbLYS1::LUZ

Plasmid

#189918

Purpose

Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.

Depositor

Adam Steinbrenner

Article

Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

Various

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-BAF

Plasmid

#227442

Purpose

Mammalian expression of GFP-BAF

Depositor

David Pellman

Article

Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

Insert

Barrier-to-autointegration factor (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-BLRR

Plasmid

#158958

Purpose

Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

BLRR

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi3-91-NLuc

Plasmid

#207150

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI3 (GNAI3 Human)

Expression

Mammalian

Mutation

NLuc inserted at position L(91)K

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi2-91-NLuc

Plasmid

#207149

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI2 (GNAI2 Human)

Expression

Mammalian

Mutation

NLuc inserted at position L(91)Q

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαq-124-NLuc

Plasmid

#207153

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAQ (GNAQ Human)

Expression

Mammalian

Mutation

NLuc inserted at position F(124)E

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gα13-125-NLuc

Plasmid

#207155

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNA13 (GNA13 Human)

Expression

Mammalian

Mutation

NLuc inserted at position F(125)D

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGfaABC1D-nLac

Plasmid

#53131

Purpose

expression of lacZ driven by human GfaABC1D promoter

Depositor

Michael Brenner

Article

Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

Insert

Gfa2 (GFAP Human)

Use

Mouse Targeting

Tags

LacZ, NLS, and mP1

Expression

Mammalian

Mutation

contains ABC1D regions of Gfa2 promoter, -1757 to…

Promoter

human Gfa2

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp16-HF

Plasmid

#157725

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp16-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pINDUCER13 (miR-LUP)

Plasmid

#46936

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp16

Plasmid

#157723

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp16 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-SUMO1

Plasmid

#185637

Purpose

expression of GST-tagged SUMO1 in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

Insert

small ubiquitin-like modifier 1 (SUMO1 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_EZH2gRNA14

Plasmid

#199586

Purpose

Contains guide RNA to 5' end of mouse EZH2 gene

Depositor

Mauro Calabrese

Article

Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

Insert

EZH2 (Ezh2 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-NMHC-IIAdeltaIQ2

Plasmid

#35690

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP and GFPGenotRW

Expression

Mammalian

Mutation

The coding segment for aa 804-834 (corresponding …

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only