We narrowed to 4,010 results for: SPL

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pXN21-Dest

Plasmid

#105086

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein

Plasmid

#208378

Purpose

Episomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split intein

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

Plasmid

#208379

Purpose

Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJYDN3

Plasmid

#162456

Purpose

Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT-Hygro hTERT FL-Firefly

Plasmid

#171057

Purpose

hTERT Full-length-expressing splicing minigene

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

hTERT FL-Firefly

Expression

Mammalian

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAXY0007

Plasmid

#205576

Purpose

NpuDnaE(C)-Glucos(232-500)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)-Glucosyltransferase

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D3

Plasmid

#204182

Purpose

Single reporter with long guanine depleted (G-poor) region from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D3

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D1.61C>G

Plasmid

#204179

Purpose

pRegev_D1 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D1 with 61C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

61C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D2.30C>G

Plasmid

#204181

Purpose

pRegev_D2 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D2 with 30C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

30C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D4.34C>G

Plasmid

#204185

Purpose

pRegev_D4 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D4 with 34C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

34C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D3.48C>G

Plasmid

#204183

Purpose

pRegev_D3 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D3 with 48C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

48C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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SPELL-GDI Component 2

Plasmid

#170487

Purpose

SPELL GDI1 C-lobe fused to FRB. Split site is 66

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB-GDI1(C-lobe)

Expression

Mammalian

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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SPELL-GDI Component 1

Plasmid

#170486

Purpose

SPELL GDI1 N-lobe fused to iFKBP. Split site is 66.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

GDI1(N-lobe)-iFKBP

Expression

Mammalian

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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plIVMD217

Plasmid

#160939

Purpose

Excess Capsid Protein in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mTagBFP2-HA Tag

Tags

HA tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) short BP-3'SS

Plasmid

#111333

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

shorter branch to 3' splice site distance

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M1

Plasmid

#106321

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc mutated splice site EEKQ-->AAAA

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M5

Plasmid

#106325

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc splice site insertion mutant EEKQNA-->EEK…

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M4

Plasmid

#106324

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc splice site deletion mutant EEKQNA-->EEKNA

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M3

Plasmid

#106323

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc mutated splice site EEKQNAV-->AAAAAAA

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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