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pRK5-mEGFP-HMGB1-delFS

Plasmid

#194555

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del FS" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

truncation by stop codon after Lys183

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-DVL1-MUT

Plasmid

#194587

Purpose

Mammalian Expression of mEGFP-DVL1 Mutant (H502Pfs*143) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

DVL1 (DVL1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

H502Pfs*143

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SOX2-MUT

Plasmid

#194581

Purpose

Mammalian Expression of mEGFP-SOX2 Mutant (M276Ifs*95) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

SOX2 (SOX2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

M276Ifs*95

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-CALR-MUT

Plasmid

#194579

Purpose

Mammalian Expression of mEGFP-CALR Mutant (D387Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

CALR (CALR Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

D387Rfs*44

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCD86-mEYFP(Q69K)-mEGFP

Plasmid

#190750

Purpose

Expression of CD86 double tagged with mEYFP(Q69K) and mEGFP in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mEGFP and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mCherry2-mEGFP

Plasmid

#182859

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mCherry2-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mEYFP-(L)-mEGFP

Plasmid

#182852

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mEYFP-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

Plasmid

#178074

Purpose

Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

MZT2A (MZT2A Human)

Use

Tags

PreScission-mEGFP and ZZ-TEV

Expression

Insect

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-C-IDR

Plasmid

#166441

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-C-IDR(266-504) (YAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-mEGFP-HDEL

Plasmid

#160467

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signal

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-mEGFP-HDEL

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

Plasmid

#114144

Purpose

The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)

Use

Tags

Expression

Mammalian

Mutation

G2A mutation and silent mutation to resist shRNA …

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mEGFP

Plasmid

#105143

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P81nmt1-mEGFP

Use

Tags

81nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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CaMKIIP-mEGFP-P2A-paAIP2/pAAV

Plasmid

#91718

Purpose

AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

Depositor

Ryohei Yasuda

Article

Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

Insert

mEGFP-P2A-paAIP2

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKII 1.3

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-BSD-tet-mEGFP-MIB1

Plasmid

#140241

Purpose

Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

Tet-inducible mEGFP-MIB1 (MIB1 Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII-ShadowG

Plasmid

#167475

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII-ShadowG

Use

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mouse PKA-Calpha-mEGFP

Plasmid

#45521

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA catalytic subunit alpha tagged by mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-WT-Full-length

Plasmid

#194548

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-B-Seipin-miniIAA7-mEGFP

Plasmid

#129719

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mEGFP

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCK-Drosha(UniProt)-FLAG-mEGFP

Plasmid

#164691

Purpose

Express Drosha-FLAG-mEGFP in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

Drosha (DROSHA Human)

Use

Tags

FLAG and mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-IDR2

Plasmid

#194552

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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