We narrowed to 9,799 results for: pET
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Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
ExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
K44A dynamin 1 pEGFP
Plasmid#34681DepositorAvailable SinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET MBP tdTomato LIC cloning vector (MBP-tdTomato)
Plasmid#86927PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-tdTomatoDepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialPromoterT7Available SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::MBP-NL3F10H
Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mKate2
Plasmid#133452PurposeExpresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mKate2
TagsGSGSGS linker and His6ExpressionBacterialPromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
spNW30
Plasmid#173485PurposeExpresses spNW30 in Bl21DepositorInsertspNW30
TagsHistagExpressionBacterialAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_BGL2
Plasmid#101911PurposeExpresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.DepositorInsertβ-glucosidase
UseSynthetic BiologyExpressionBacterialPromoterT7+LacOAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
spNW50
Plasmid#173486PurposeExpresses spNW50 in Bl21DepositorInsertspNW50
TagsHistagExpressionBacterialAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Kras-Src FRET biosensor
Plasmid#78302PurposeFRET biosensor. To monitor Src kinase activity in mammalian cells by FRETDepositorInsertSrc biosensor
ExpressionMammalianPromoterCMVAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-d2eGFP-cxcr4
Plasmid#21967DepositorTypeEmpty backboneExpressionMammalianAvailable SinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCT310
Plasmid#111140PurposeBacterial expression of 6His-dCas9-3XFlagDepositorInsertdCas9
Tags3X Flag and 6X HisExpressionBacterialMutationD10A - H840APromoterT7Available SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
CS2 Flag-Smad6
Plasmid#14961DepositorAvailable SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO mouse shRNA 1 raptor
Plasmid#21339DepositorAvailable SinceJuly 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
spNW15
Plasmid#173483PurposeExpresses spNW15 in Bl21DepositorInsertspNW15
TagsHistagExpressionBacterialAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
wt dynamin 1 pEGFP
Plasmid#34680DepositorAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHMM
Plasmid#67717PurposeExpression vector MBP/hexahistidine tagged variant of the MS2 coat proteinDepositorInsertHexahistidine/Maltose binding protein/MS2 coat protein fusion
TagsHexahistidine tagExpressionBacterialPromoterT7 RNA polymeraseAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDB302
Plasmid#113673Purposeexpresses Cth SUMO protease in Escherichia coliDepositorInsertCth SUMO protease
Tags10-HisExpressionBacterialMutationdeleted amino acids 1-934PromoterT7Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-β-Lactamase-SpyCatcher
Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
TagsSpyCatcher and SpyTagExpressionBacterialPromoterT7 promoterAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHN924
Plasmid#178885PurposeExpression of GST-His8-HRV3CDepositorInserthuman rhinovirus 3C protease
TagsGST, His8ExpressionBacterialPromoterT7Available SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only