We narrowed to 14,883 results for: pET

1. pET15-ZmPYK

   Plasmid

   #220230

   Purpose

   Inducible expression of Zymomonas mobilis pyruvate kinase

   Depositor

   Liskin Swint-Kruse, Aron Fenton

   Article

   Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

   Insert

   Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15TV-L-AldA WT

   Plasmid

   #187976

   Purpose

   Expresses human aldolase A

   Depositor

   Audrey Lamb

   Article

   Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

   Insert

   AldA WT (ALDOA Human)
   

   Use

   Tags

   Hexahistag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a AHU

   Plasmid

   #26640

   Depositor

   Phoebe Rice

   Article

   Swinger et al EMBO J. 2003 Jul 15. 22(14):3749-60.

   Insert

   AHU
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-cMSP1E3D1

   Plasmid

   #199772

   Purpose

   Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

   Insert

   cMSP1E3D1
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Extended cMSP1D1; external His-Tags flanking the …

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29b-pduCDE

   Plasmid

   #110138

   Purpose

   Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

   Insert

   pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET30B-PTEN

   Plasmid

   #20741

   Depositor

   Alonzo Ross

   Article

   Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pET30-RT1306

   Plasmid

   #131521

   Purpose

   Expresses engineered RT-1306 with His-tag

   Depositor

   Bryan Dickinson

   Article

   Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

   Insert

   RT-1306
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET303-colE1T

   Plasmid

   #155180

   Purpose

   Expresses colicin E1 residues 1-190

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   colicin E1 residues 1-190
   

   Use

   Tags

   C-terminal 6x Histidine tag

   Expression

   Bacterial

   Mutation

   truncation of colicin E1 including 1-190

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-KSRP

   Plasmid

   #23019

   Depositor

   Douglas Black

   Article

   Min et al Genes Dev. 1997 Apr 15. 11(8):1023-36.

   Insert

   KSRP (KHSRP Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET24b-BG1.3

    Plasmid

    #199437

    Purpose

    expresses BAmGreen1.3 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen1.3
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-H12TEV

    Plasmid

    #208635

    Purpose

    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-BE1

    Plasmid

    #73018

    Purpose

    Expresses BE1 with N-terminal His6 tag in E. Coli

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11a-hRanGAP1

    Plasmid

    #50941

    Purpose

    expression of full length untagged human RanGAP1 in E.coli

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanGTPase activating protein 1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET24b-BR1.4

    Plasmid

    #199436

    Purpose

    expresses BAmRed1.4 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMRed1.4
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET24b-Amg2

    Plasmid

    #199434

    Purpose

    expresses Amg2 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    Amg2
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    L159K mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-MKH8SUMO

    Plasmid

    #79526

    Purpose

    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pET28a-Rx3(A7)

    Plasmid

    #35525

    Depositor

    Robert Cohen

    Article

    Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

    Insert

    His6-Rx3(A7)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Changed G2 to P

    Promoter

    T7

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET32-MCM2/7

    Plasmid

    #116949

    Purpose

    Expresses human MCM2 and human MCM7

    Depositor

    James Chong

    Article

    Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

    Inserts

    Human MCM7 (MCM7 Human)
    Human MCM2 (MCM2 Human)
    

    Use

    Tags

    Thioredoxin, His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM33_PDCD6IP_ALIX V-domain

    Plasmid

    #178483

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    PDCD6IP_ALIX V-domain (PDCD6IP Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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