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pET28a-TRBP

Plasmid

#50351

Purpose

bacterial expression of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

TRBP (TARBP2 Human)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pET3a-Yuh1

Plasmid

#18895

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Ubiquitin C-terminal hydrolase I (YUH1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pET30-2-PSAT1

Plasmid

#83908

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

phosphoserine aminotransferase 1 (PSAT1 Human)

Use

Tags

6x His tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pET-X(5010)B1

Plasmid

#168019

Purpose

Expresses X(5010)B1-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(5010)B1-6xHis

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET11d-RAN

Plasmid

#108919

Purpose

Expresses human RAN

Depositor

Larry Gerace

Article

Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

Insert

RAN (RAN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET3a-Ub-K63R

Plasmid

#18898

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Ubiquitin-K63R (UBC Human)

Use

Tags

Expression

Bacterial

Mutation

Lysine 63 is replaced with a Argnine residue

Promoter

Available since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pET21b-P35

Plasmid

#11808

Depositor

Guy Salvesen

Article

Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-8

Insert

P35

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
+36GFP-LPETG

Plasmid

#71754

Purpose

+36GFP-LPETG for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_(pos36GFP)_6XHis_LPETG

Use

Tags

6XHis and Sortase (LPETG)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET-CV-SNAP25

Plasmid

#168031

Purpose

Expresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-SNAP25(A141-G206)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-pduCDE

Plasmid

#110138

Purpose

Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

Insert

pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1delltaH4

Plasmid

#71713

Purpose

Membrane scaffold protein (MSP), helix 4 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pET11d-hUba2

Plasmid

#53136

Purpose

E.coli expression of full length untagged human Uba2 (SAE2)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

UBA2 (UBA2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET-X(4130)B1

Plasmid

#168018

Purpose

Expresses X(4130)B1-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(4130)B1-6xHis

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX

Plasmid

#111516

Purpose

Expresses E. coli ClpX with a C-terminal His tag

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Use

Tags

10xHis-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-mNG-NL

Plasmid

#169106

Purpose

Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - mNeonGreen - NanoLuc

Use

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET20b_Pa-PiuA

Plasmid

#128955

Purpose

expresses Pseudomonas aeruginosa PiuA in E. coli

Depositor

James Naismith

Article

Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

Insert

iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)

Use

Tags

6xHis and pelB leader

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-FOXP2_P6

Plasmid

#122649

Purpose

Expresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pYPet-His

Plasmid

#14031

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

Insert

YPet

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pET28-hTDG

Plasmid

#70758

Purpose

bacterial expression of human TDG

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

TDG (TDG Human)

Use

Tags

6-HIS

Expression

Bacterial

Mutation

Promoter

T7/lac operon

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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