We narrowed to 54,949 results for: ran

1. ClhN-MET15

   Plasmid

   #207013

   Purpose

   Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ClhN-URA3

   Plasmid

   #207012

   Purpose

   Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-AGG-NLS-EGFP

   Plasmid

   #214602

   Purpose

   express EGFP ; in vitro transcribed modified RNA vector

   Depositor

   Yuxuan Guo

   Article

   Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

   Insert

   EGFP
   

   Use

   Unspecified

   Promoter

   T7

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-AGG-SpCas9

   Plasmid

   #214603

   Purpose

   encode SpCas9; in vitro transcribed modified RNA vector

   Depositor

   Yuxuan Guo

   Article

   Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

   Insert

   SpCas9
   

   Use

   CRISPR and Unspecified

   Promoter

   T7

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. BS-TRP1-PGAL-GFP-Cps1

   Plasmid

   #207030

   Purpose

   Expression of GFP-Cps1 under GAL promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Cps1
   

   Tags

   GFP

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCUP1-4V5-Pho8-Met

   Plasmid

   #207029

   Purpose

   Expression of 4V5-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Tags

   4xV5

   Expression

   Yeast

   Promoter

   pCUP1

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. BS-Trp-PCUP1-4myc-Pho8

   Plasmid

   #207028

   Purpose

   Expression of 4Myc-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Tags

   4xMyc

   Expression

   Yeast

   Promoter

   pCUP1

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PCUP1-Vac8-GFP-Ura

   Plasmid

   #207027

   Purpose

   Expression of Vac8-GFP under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Vac8
   

   Tags

   GFP

   Expression

   Yeast

   Promoter

   pCUP1

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PCUP1-GFP-Vma13-Ura

    Plasmid

    #207026

    Purpose

    Expression of GFP-Vma13 under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Vma13 ORF
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    pCUP1

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PCUP1-mRuby3-Pho8-406

    Plasmid

    #207043

    Purpose

    Expression of mRuby3-Pho8 under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    PHO8
    

    Tags

    mRuby3

    Expression

    Yeast

    Promoter

    pCUP1

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. P1K-3HA-Atg8∆R-MET15

    Plasmid

    #207034

    Purpose

    Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆R

    Promoter

    pATG8

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42229

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42337

    Purpose

    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. BS-TRP1-TetOff(7repeats)-GFP-Pho8

    Plasmid

    #207011

    Purpose

    Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PADH1-TIR1-9myc-URA3

    Plasmid

    #207016

    Purpose

    Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    TIR1
    

    Tags

    9xMyc

    Expression

    Yeast

    Promoter

    pADH1

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BS-ZJLEU-PTEF1-GFP

    Plasmid

    #207018

    Purpose

    Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    GFP
    

    Expression

    Yeast

    Promoter

    pTEF1

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PTPI1-Dsred-URA3

    Plasmid

    #207017

    Purpose

    Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    DsRed-Express 2
    

    Expression

    Yeast

    Promoter

    pTPI1

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

    Plasmid

    #207024

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A Y387A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

    Plasmid

    #207023

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A I375A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge