We narrowed to 10,856 results for: nar;

1. sgRNA scaffold-spacer-human U6

   Plasmid

   #214697

   Purpose

   provide "sgRNA scaffold-spacer-human U6" fragment

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   sgRNA scaffold-spacer-human U6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcGP67A-rCL1-HormR-GAIN

   Plasmid

   #37847

   Depositor

   Axel Brunger

   Article

   Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

   Insert

   CL1 (Adgrl1 Rat)
   

   Use

   Baculovirus transfer vectors

   Tags

   8xHIS

   Expression

   Mutation

   HormR and GAIN domains only (aa460–849

   Promoter

   Polyhedrin

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pBEAST-pBen-sfGFP

   Plasmid

   #114598

   Purpose

   "Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   PBen

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEAST-BenR

   Plasmid

   #114597

   Purpose

   "Strong constitutive expression of transcription factor, BenR, for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   BenR
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Stx3-pmCitrine

   Plasmid

   #92421

   Purpose

   Expression of syntaxin-3 C-terminally conjugated to mCitrine.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   Stx3 (Stx3 Rat)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDendra2-Hygro

   Plasmid

   #202407

   Purpose

   Dendra2 expression plasmid

   Depositor

   Morito Kurata

   Article

   Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

   Insert

   Hygromycin
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PLEX_313 GFP

   Plasmid

   #184494

   Purpose

   Lentivirus expression for GFP

   Depositor

   Peter Tsvetkov, Todd Golub

   Article

   Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Lyn-AktAR2

   Plasmid

   #175013

   Purpose

   Plasma membrane-targeted AktAR2.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   Lyn-mCherry-Akt-STOPS
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQZ083

   Plasmid

   #106417

   Purpose

   Express Complexin-1 fragment (1-83) in E. coli

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

   Insert

   complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Fragment 1-83, Codon optimization

   Promoter

   T7

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pGAD-Atg17

    Plasmid

    #121386

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcCas12nHS_RV6_PT1_empty

    Plasmid

    #203810

    Purpose

    Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12nHS_RV6_PT1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-28a-His6-SUMO-VAMP2

    Plasmid

    #135551

    Purpose

    Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP2
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRRG304-Ndk

    Plasmid

    #99072

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea Ndk (nou darake)

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

    Insert

    Ndk (nou darake) in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX 4t1 RARalpha (1-187)

    Plasmid

    #35558

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 1-187

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pGEX 4T1 RARalpha (186-462)

    Plasmid

    #35559

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 186-462

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

17. CAGhCBFA2T2

    Plasmid

    #84363

    Purpose

    FLAG-HA tagged human CBFA2T2

    Depositor

    Danny Reinberg

    Article

    Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

    Insert

    CBFA2T2 (CBFA2T2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRV DYRK2

    Plasmid

    #12568

    Depositor

    Anjana Rao

    Article

    Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

    Insert

    DYRK2 (DYRK2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pRRG227-Wnt1

    Plasmid

    #99073

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea Wnt1 (also known as WntP-1)

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

    Insert

    Wnt1 (also known as WntP-1) in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBEAST-J23101-CocE

    Plasmid

    #114600

    Purpose

    "Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    CocE
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    J23101

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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