We narrowed to 22,649 results for: promoter

1. pFPV25-cR-2iG

   Plasmid

   #204618

   Purpose

   Fluorescent reporter for SPI2 induction in Salmonella

   Depositor

   Jacques Neefjes

   Article

   Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

   Inserts

   rpsM promoter
   sseA promoter
   

   Use

   Tags

   GFP and dsRed

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJR106

   Plasmid

   #187244

   Purpose

   Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. His10-CD3z-SNAPf-ybbR (XSB507)

   Plasmid

   #78508

   Purpose

   Construct for expressing CD3z-SNAPf-ybbR in E. Coli

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   CD3z (Cytoplasmic domain) (CD247 Human)
   

   Use

   Tags

   His10 and SNAPf-ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT-sox10-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80063

   Purpose

   Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Sox10 promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe_Zeo_cOVA_T2A_mStrawberry

   Plasmid

   #161736

   Purpose

   Expression of cOVA and mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   cytoplasmic ovalbumin
   

   Use

   Retroviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti-px330-CRBN-T1-pGK-Pur

   Plasmid

   #107382

   Purpose

   Mammalian expression CRISPR/Cas9

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   sgRNA targeting CRBN (CRBN Human)
   

   Use

   Lentiviral; Crispr

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5 HA-TBRII (delta Cyt)

   Plasmid

   #14051

   Depositor

   Joan Massague

   Article

   Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

   Insert

   TGF beta rec II (delta cyt) (TGFBR2 Human)
   

   Use

   Tags

   HA (PYDVPDYA)

   Expression

   Mammalian

   Mutation

   Dominant negative mutant. A stop codon was introd…

   Promoter

   Available since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR1a-tagRFP-KDEL

   Plasmid

   #114177

   Purpose

   Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   tagRFP-KDEL
   

   Use

   Gateway entry clone

   Tags

   KDEL retention signal and signal peptide

   Expression

   Mutation

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5E TRE-3GV (JDW 1163)

   Plasmid

   #224476

   Purpose

   Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   TRE3GV promoter
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. NSP6-Flag

    Plasmid

    #210345

    Purpose

    Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP6 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBbsr2-Gnaq

    Plasmid

    #170298

    Purpose

    Encoding mouse Gnaq

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A recombinant mouse Gnaq (Gnaq Mouse)
    

    Use

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Asilent mutation was introduced into the sgRNA-ta…

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry

    Plasmid

    #122957

    Purpose

    Lentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NLB (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xmyc tag

    Expression

    Mutation

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pATP416-­pluxO5-­pphlO6-­crtYBI

    Plasmid

    #165977

    Purpose

    Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pluxO5), Synthetic promoter (…

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMG046 pETARA-CK1α-His

    Plasmid

    #154074

    Purpose

    Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-SIRT2-Flag

    Plasmid

    #102624

    Purpose

    Expresses full length SIRT2 isoform 1 in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFE-Rrub-FII-prk

    Plasmid

    #162695

    Purpose

    Expresses R. rubrum Form II rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Inserts

    Rubrum Form II rubisco
    S. elongatus PRK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter and under same promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR

    Plasmid

    #171596

    Purpose

    Ectodomain deleted negative control ACE2 from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    ACE2 (ACE2 Human)
    mCherry
    H2A
    Pac
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

    Plasmid

    #139995

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    HF1=N497A/R661A/Q695A/Q926A

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRIGF-IKZF3

    Plasmid

    #69046

    Purpose

    expression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    IRES

    Available since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag-Rabin8

    Plasmid

    #118070

    Purpose

    Expresses Flag-tagged human Rabin8 in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    human Rabin8 (RAB3IP Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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