We narrowed to 9,345 results for: sacs
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Plasmid#164573PurposeIntegration of 2 LoxN sites in the genome of P. bergheiDepositorInsertsUseCre/LoxPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only
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BAS285 C terminal mKO2 hygBR
Plasmid#74080PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB resistance.DepositorInsertmKO2
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1ExpressionYeastAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB3035(X-4 MarkerFree)
Plasmid#73273PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDNA3-Cerulean-FHA1-NES
Plasmid#138202PurposeEncodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.DepositorInsertCerulean-FHA1-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP2279
Plasmid#70706PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A and H557A in SaCas9PromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GW-dCas9-SET2-IRES2-mCherry
Plasmid#202089PurposeNeuron-specific expression vector of dCas9-Set2DepositorInsertdCas9-Set2
Tags3x FLAG and mCherryExpressionMammalianPromoterhuman SynapsinAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCT
Plasmid#60620PurposePlasmid encoding iCas9 and tracrRNA on pRS415 backboneDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
prp8-G736E
Plasmid#108450Purposeprp8-G736E in pRS313 backbone for expression in yeastDepositorAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MmSphk2
Plasmid#118598PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB3034(X-3 MarkerFree)
Plasmid#73272PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
YEpURA-CUP1-HBTubiquitin
Plasmid#26900DepositorInsertHBT-tagged ubiquitin
ExpressionYeastAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1
Plasmid#159166PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1
TagsSV40 NLSExpressionYeastPromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
POT4-RFP
Plasmid#65320Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
BAS288 C terminal mKO2 natR
Plasmid#74083PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.DepositorInsertmKO2
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPMVAugw
Plasmid#98300PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.DepositorInsertTEFM1EfmvaE>TEBS1
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
ExpressionYeastPromoterCYC1Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 mito roGFP2-Orp1
Plasmid#65001PurposeExpression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1-NES
Plasmid#84632PurposeUnimolecular, FRET-based JNK activity reporter; cytosol targetedDepositorInsertECFP-FHA1-JNKsubDD-Citrine-NES
Tags6xHis, Citrine, ECFP, Nuclear export signal (NES)…ExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2803
Plasmid#63646PurposepCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only