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pGLUE-SHLD2

Plasmid

#114118

Purpose

Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Tags

HA tag, Streptavidin-binding tag (Streptag), and …

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pTRIP-SFFV-mtagBFP-2A STING-354STOP

Plasmid

#102589

Purpose

Lentivector to express STING delta 354 and mTagBFP

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta 354

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78304

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Publication

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Expression

Mammalian

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pME-nlsGal80(opt)

Plasmid

#51618

Purpose

vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

Depositor

Joshua Bonkowsky

Publication

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gal80(opt) (GAL80 Budding Yeast)

Use

Vertebrate expression

Tags

NLS

Mutation

codon optimized

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-EPHX1

Plasmid

#53114

Purpose

In vitro translation of microsomal epoxide hydrolase

Depositor

Xu Lin

Publication

Chen et al BMC Cancer. 2014 Apr 23;14:282. doi: 10.

Insert

EPHX1 (EPHX1 Human)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only
pRSCgfp-hAIM2

Plasmid

#51666

Purpose

Express GFP and hAIM2 in mammalian cells

Depositor

Emad Alnemri

Publication

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

AIM2 (AIM2 Human)
GFP

Expression

Mammalian

Promoter

CMV and RSV

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-N1-zebrafish lyn–GFP

Plasmid

#35956

Depositor

Anna Huttenlocher

Publication

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized zebrafish lyn (lyn Zebrafish)

Tags

EGFP

Expression

Mammalian

Mutation

wt

Promoter

cmv

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV FHA-Stop

Plasmid

#27050

Depositor

Jayanta Debnath

Publication

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Mutation

changed glycine 141 to stop codon.

Available since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRF+275Dux4

Plasmid

#21293

Depositor

Stephen Tapscott

Publication

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DUX 4 (DUX4 DUX 4, Human)

Use

Luciferase

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
LB595 (Bcl-2 promoter CRE mut)

Plasmid

#15243

Depositor

Linda Boxer

Publication

Heckman et al Oncogene. 2002 May 30. 21(24):3898-908.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Mutated CRE site at -1537 changed from GTGACGTTA …

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-syn-iGluSnFR4f-PDGFR-WPRE

Plasmid

#234436

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Publication

Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi

Insert

iGluSnFR4f-PDGFR

Use

AAV

Tags

IgK chain and Myc epi tag-PDGFR TM Domain

Expression

Mammalian

Promoter

Synapsin

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1_puro_shNRF2-1

Plasmid

#230086

Purpose

shRNA silencing human NRF2 gene

Depositor

Xingguo Zhu

Publication

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherryC1 VAP-B

Plasmid

#226409

Purpose

Expression of human VAP-B fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Publication

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VAP-B (VAPB Human)

Tags

HA, T7-Xpress tag, and mCherry

Expression

Mammalian

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pKb-TnpB1

Plasmid

#215333

Purpose

ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

Depositor

Kutubuddin Molla

Publication

Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

Insert

Rice codon optimized ISDra2 TnpB

Tags

Not applicable

Expression

Plant

Promoter

Rice ubiquitin promoter for TnpB and OsU3 promote…

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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PCDH-Mut-ALKBH5-3xFLAG

Plasmid

#203737

Purpose

Overexpression of ALKBH5-Mut

Depositor

Jianjun Chen

Publication

Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

Insert

ALKBH5 (ALKBH5 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Enzyme Inactive Mutation (H226A)

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-mScarlet-Giantin

Plasmid

#196872

Purpose

Expresion of Giantin-fused to mScarlet. Used as Golgi marker

Depositor

Frank Bradke

Publication

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet-Giantin (GOLGB1 Synthetic, Human)

Expression

Mammalian

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHSG1C3

Plasmid

#164423

Purpose

single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.

Depositor

Xiao Wang

Publication

Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ePB-PURO-FLAG-METTL3-APPA

Plasmid

#160253

Purpose

For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Publication

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

tight TRE promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-PKCg-mEGFP-N1

Plasmid

#112270

Purpose

IDOCKSgamma Donor Construct

Depositor

Ryohei Yasuda

Publication

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

protein kinase c gamma (Prkcg Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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