We narrowed to 9,174 results for: Pol

1. pET28_6xHis-PARP1delZn1-L713F

   Plasmid

   #173937

   Purpose

   Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn1-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 1-96; L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1-G871L

   Plasmid

   #174798

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G871L (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G871L, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_6xHis-PARP1-A870L

   Plasmid

   #174797

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A870L (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A870L, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_6xHis-PARP1-K893I

   Plasmid

   #174799

   Purpose

   Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-K893I (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K893I, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKHH030_sgCtrl#2

   Plasmid

   #174143

   Purpose

   Lentiviral vector expressing a control sgRNA that targets a safe harbor site

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX026

   Plasmid

   #167153

   Purpose

   MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   p35s-nnLuz-ocsT
   

   Use

   Luciferase

   Tags

   His6-tag

   Expression

   Plant

   Promoter

   p35s

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR_P4-P1R:PER36modp

   Plasmid

   #128431

   Purpose

   Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).

   Depositor

   George Haughn

   Article

   McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

   Insert

   PEROXISADE36 (AT3G50990 Mustard Weed)
   

   Use

   Gateway promoter entry clone

   Mutation

   Premature stop codons introduced into the Xero1 (…

   Promoter

   PEROXISADE36

   Available Since

   Oct. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pJC20cdc8.A18T

   Plasmid

   #99360

   Purpose

   Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   Alanine 18 to Threonine

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJC20cdc8.E31K

   Plasmid

   #99361

   Purpose

   Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   Glutamic acid 31 to Lysine

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJC20cdc8.110

    Plasmid

    #99363

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine and Glutamic acid 31 to L…

    Promoter

    T7

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-GST-CHD5-L518A/Y619E-FRAG

    Plasmid

    #68874

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    331-672aa, L518A/Y619E

    Promoter

    tac

    Available Since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

12. hKCNQ5(WT).ires.GFP-pcDNA3

    Plasmid

    #204360

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. hKCNQ3(WT).ires.mCherry-pcDNA3

    Plasmid

    #204362

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. hBACE1(D289N).ires.mCherry-pcDNA3

    Plasmid

    #204363

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    BACE1 (BACE1 Human)
    

    Expression

    Mammalian

    Mutation

    D289N

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. hKCNQ5(WT).ires.mScarlet-pcDNA3

    Plasmid

    #204361

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 SARS-CoV2 TEV-NSP12

    Plasmid

    #154410

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. hKCNQ5(K289N).ires.GFP-pcDNA3

    Plasmid

    #204364

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    K289N

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. hKCNQ5(P369T).ires.GFP-pcDNA3

    Plasmid

    #204365

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    P369T

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. hKCNQ5(R443Q).ires.GFP-pcDNA3

    Plasmid

    #204366

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    R443Q

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. hKCNQ5(H576R).ires.GFP-pcDNA3

    Plasmid

    #204367

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    H576R

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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