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pSR1055

Plasmid

#208741

Purpose

Characterization plasmid for PHrcA (sfgfp expressed from PHrcA on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PHrcA

Use

Synthetic Biology

Expression

Bacterial

Promoter

PHrcA

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-MBP_D

Plasmid

#146120

Purpose

Insect Expression of MBP

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

MBP

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-mEGFP-HDEL

Plasmid

#160467

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signal

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-mEGFP-HDEL

Expression

Yeast

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-S*HiGlpG

Plasmid

#158534

Purpose

Bacterial expression of rhomboid protease mutant S*HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

S*HiGlpG

Tags

GST tag

Expression

Bacterial

Mutation

Sector 1 and 2 mutations

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-S1*HiGlpG

Plasmid

#158535

Purpose

Bacterial expression of rhomboid protease mutant S1*HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

S1*HiGlpG

Tags

GST tag

Expression

Bacterial

Mutation

Sector 1 mutations

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-S2*HiGlpG

Plasmid

#158536

Purpose

Bacterial expression of rhomboid protease mutant S2*HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

S2*HiGlpG

Tags

GST tag

Expression

Bacterial

Mutation

Sector 2 mutations

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPRi-CENPB-g4

Plasmid

#120212

Purpose

CENPB CRISPRi guide RNA 4

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPRi-CENPB-g3

Plasmid

#120211

Purpose

CENPB CRISPRi guide RNA 3

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-ILVBLpA-G10C

Plasmid

#120206

Purpose

Human ILVBL poly(A) site reporter, stem loop mutant

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

ILVBL poly(A) site

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-del

Plasmid

#120200

Purpose

human RPS5 poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m2r

Plasmid

#120199

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2 rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m2

Plasmid

#120198

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m1r

Plasmid

#120197

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 1 rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m1

Plasmid

#120196

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 1

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-CENPBpA-del

Plasmid

#120193

Purpose

human CENPB poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-TFE3pA-del

Plasmid

#120204

Purpose

human TFE3 poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-TFE3pA-G9C

Plasmid

#120202

Purpose

Human TFE3 poly(A) site reporter, stem loop mutant

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPRi-CENPB-g10

Plasmid

#120218

Purpose

CENPB CRISPRi guide RNA 10

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPRi-CENPB-g9

Plasmid

#120217

Purpose

CENPB CRISPRi guide RNA 9

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPRi-CENPB-g8

Plasmid

#120216

Purpose

CENPB CRISPRi guide RNA 8

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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