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pWR23

Plasmid

#202770

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coKanMX
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR10

Plasmid

#202710

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coShBle
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR9

Plasmid

#202645

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1_mNeonGreen_Ptet-2_mCherry

Plasmid

#213839

Purpose

Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen, mCherry

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_TetR

Plasmid

#213847

Purpose

Facilitates genomic integration of tetR expression cassette.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

TetR

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-OM14-V5-APEX2-Ura

Plasmid

#207073

Purpose

Expressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

OM14

Tags

V5-APEX2

Expression

Yeast

Promoter

pOM14

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTcI38-PRM-wasabi

Plasmid

#195554

Purpose

Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
TcI38

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T, M1V, L65S, K68R, F115L, D126G, D188G

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCIwt-PRM-Wasabi

Plasmid

#195553

Purpose

Always-on expression of mWasabi reporter with transcription activation by wildtype CI

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
CIwt

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGGC-PEA3AT

Plasmid

#210536

Purpose

Cloning P. sativum PEA3A terminator as GreenGate module C

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

PEA3At

Use

Synthetic Biology

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGGA-pCLV3

Plasmid

#210528

Purpose

Cloning A. thaliana CLV3 promoter as GreenGate module A

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pCLV3 (CLV3 Mustard Weed)

Use

Synthetic Biology; Golden gate compatible cloning…

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-GFP-APEX2-Ura

Plasmid

#207062

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Tgl3-4V5-APEX2-URA

Plasmid

#207059

Purpose

Expressing Tgl3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pTGL3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Pex3-4V5-APEX2-URA

Plasmid

#207057

Purpose

Expressing Pex3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

PEX3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pPEX3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_HD_G1333-DddA11'-C

Plasmid

#199797

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NG_G1333-DddA11'-C

Plasmid

#199798

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NI_G1333-DddA11'-C

Plasmid

#199799

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NN_G1333-DddA11'-C

Plasmid

#199800

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_HD_G1333-DddA11'-N

Plasmid

#199801

Purpose

ptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NG_G1333-DddA11'-N

Plasmid

#199802

Purpose

ptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NI_G1333-DddA11'-N

Plasmid

#199803

Purpose

ptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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