We narrowed to 13,292 results for: cDNA

1. 3341 pcDNA3 Bcl-2 T69A S70A S87A

   Plasmid

   #13340

   Depositor

   Stanley Korsmeyer

   Article

   Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 69 to Alanine; changed Serine 7…

   Promoter

   Available Since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3 H2B-mIFP T2A Mpro (C145A)

   Plasmid

   #163080

   Purpose

   Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

   Depositor

   Xiaokun Shu

   Insert

   Inactive Mpro mutant C145A and H2B-mIFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

   Plasmid

   #121976

   Purpose

   transfection studies in eukaryotic cells such as COS-7 cells

   Depositor

   Christophe Erneux

   Article

   De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

   Insert

   Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. (318) pcDNA3.1-myc-OGA(1-400)D174N

   Plasmid

   #168096

   Purpose

   Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   myc-OGA(1-400, D174N)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   D174N catalytically inactive OGA

   Promoter

   T7/CMV

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-TAT-1-101-FLAG basic mutant

   Plasmid

   #35985

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV1, 101 aa; R to A mutations at aa 56,…

   Promoter

   Available Since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

   Plasmid

   #163988

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   M1457A

   Promoter

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

   Plasmid

   #164845

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
   

   Use

   Tags

   8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression. Has N5…

   Promoter

   CMV

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

   Plasmid

   #176060

   Purpose

   Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K164A and H165A

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

   Plasmid

   #125796

   Purpose

   for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

   Depositor

   P. Chen

   Article

   Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

   Insert

   CD40L (lose of function mutant)-mgSrtA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

    Plasmid

    #136603

    Purpose

    Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    FKBP-C-mSOG-J-alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-S28 histone 3 phosphorylation reporter-NLS

    Plasmid

    #32787

    Depositor

    Alice Ting

    Article

    Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

    Insert

    CFP-14-3-3tau-H3 peptide-YFP
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid

    #216292

    Purpose

    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras heavy chain-GSG-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

    Plasmid

    #166804

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S1213A

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194377

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

    Plasmid

    #176059

    Purpose

    Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST

    Plasmid

    #194385

    Purpose

    Gateway Destination vector for split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    attR1-ORF-attR2-NTEV-TCS-GV-2xHA
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Hygro(+)-PSTCD-cGAS-delta DBD

    Plasmid

    #102606

    Purpose

    Mammalian expression of mutant cGAS with deletion of DNA binding domain fused to PSTCD tag

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta K173-I220 and delta H390-C405

    Promoter

    CMV

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3puro-mGFP-FXR1a-Ccmut (N202P-V361P)

    Plasmid

    #225457

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a (N202P V361P)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N202P V361P

    Promoter

    CMV

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

    Plasmid

    #200980

    Purpose

    Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Use

    Tags

    IgG1 Fc

    Expression

    Mammalian

    Mutation

    Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1357 pcDNA3 flag FKHR AAA delta DB

    Plasmid

    #10696

    Depositor

    William Sellers

    Insert

    FKHR AAA deltaDB (FOXO1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    T24A S256A S319A , deletion in aa 208-220

    Promoter

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only