We narrowed to 14,239 results for: cas9

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8xCTS1_delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

Plasmid

#124545

Purpose

Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro

Expression

Mammalian

Mutation

d11-41, d11-41

Promoter

Minimal ADE

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pIK137

Plasmid

#65632

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment

Expression

Worm

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pORANGE Cacnb2-GFP KI

Plasmid

#139661

Purpose

Endogenous tagging of CaVβ2: C-terminal (amino acid position: Q655)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAP2050-2

Plasmid

#105243

Purpose

mammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

GFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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gh242

Plasmid

#106850

Purpose

expression of gRNA targeting PIGA

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

PIGA (PIGA Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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sgRNA4_NANOG

Plasmid

#64156

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAP2042-1

Plasmid

#105244

Purpose

mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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sgRNA3_NANOG

Plasmid

#64155

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA3_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA2_NANOG

Plasmid

#64154

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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gh173

Plasmid

#106834

Purpose

expression of gRNA targeting UGGT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

UGGT2 (UGGT2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh127

Plasmid

#106797

Purpose

expression of gRNA targeting XYLT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

XYLT1 (XYLT1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh128

Plasmid

#106798

Purpose

expression of gRNA targeting XYLT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

XYLT2 (XYLT2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-LRTM2

Plasmid

#69237

Purpose

U6 driven SpCas9 sgRNA expression for LRTM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

LRTM2 sgRNA (LRTM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11061

Plasmid

#68255

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-1

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pPD684 EnAsCas12a-BFP

Plasmid

#249157

Purpose

Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

EnAsCas12a-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAcr

Plasmid

#207570

Purpose

AcrIIC1-mediated inhibition of ThermoCas9 and GeoCas9 cleavage activity in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Prha_AcrIIC1Nme

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pADH100Cau

Plasmid

#244817

Purpose

Modified plasmid from pADH100 to perform HIS-FLP CRISPR in Candidozyma auris

Depositor

Patrick Van Dijck

Article

Sofras et al bioRxiv [Preprint]. 2025 Jan 11:2025.01.

Insert

SNR52p from C. auris

Use

CRISPR

Tags

HIS1 terminator from C. auris and NAT resistance …

Expression

Yeast

Promoter

SNR52p

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGRF-GIF

Plasmid

#243677

Purpose

Expresses a chimeric protein composed of the wheat GROWTH-REGULATING-FACTOR and its wheat cofactor GRF-INTERACTING-FACTOR1 in plants via the CmYLCV 9.11 promoter and an Arabidopsis HSP terminator

Depositor

Patrick Krysan

Article

De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.

Insert

Chimeric protein composed of GROWTH-REGULATING-FACTOR and its cofactor GRF-INTERACTING-FACTOR1 from wheat

Expression

Plant

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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iSM-IP

Plasmid

#235990

Purpose

Expresses iSM (iSpyMac) in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM

Use

CRISPR

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-Z3-Nat-ADE2

Plasmid

#232106

Purpose

Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

Z3

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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