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Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterCAGAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCB-2
Plasmid#210388PurposeAll-in-one cumate-based inducible CRISPRi plasmid for use in StreptomycesDepositorInsertsdCas9
CymR
sgRNA cassette
UseCRISPR; Integrative vector for use in streptomycesMutationBsaI-flanked RFP cassette inserted in place of sp…PromoterSP11 and SP30Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaGustducin-RLuc8
Plasmid#140979PurposeEncodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaGustducin-RLuc8 (GNAT3 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human SHIP1 (clone hp51CN)
Plasmid#123347PurposeExpression of human SHIP1DepositorInsertINPP5D (INPP5D Human)
ExpressionMammalianAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pclbw-eGFPomp25
Plasmid#58424PurposeMammalian expression of GFP targeted to the mitochondrial outer membraneDepositorInserteGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25PromoterCMV/b-actinAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPR - EGFP sgRNA 4
Plasmid#51763PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 4
UseCRISPR and LentiviralExpressionMammalianPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YFP-Rac2(N17)
Plasmid#11398DepositorInsertras-related C3 botulinum toxin substrate 2 (RAC2 Human)
TagsYFPExpressionMammalianMutationObtained with Threonine 17 changed to Asparagine.Available sinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
p255 pPr-CREM/Ins/CMV-STOP-luc
Plasmid#8408PurposeModified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.DepositorInsertCREM/insulators/lox/luciferase
UseCre/LoxExpressionMammalianMutationmodified CrePromoterProbasin, CMVAvailable sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDONR-53BP1 1-1972 28A
Plasmid#53447PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGateway CloningMutationI914T mutation; All 28 SQ/TQ motifs in N-term of …Available sinceDec. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLTR PKC delta
Plasmid#8419DepositorInsertMouse Protein Kinase C delta (Prkcd Mouse)
ExpressionMammalianAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-iPE-Plus
Plasmid#214062Purposeinducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locusDepositorInsertPE-Plus
ExpressionMammalianPromoterTRE-tightAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK FKBP12(F36V)-OGT (Neo)
Plasmid#154294PurposeLentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoterDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x HA and FKBP12(F36V)ExpressionMammalianPromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6xHis-TagRFP-T_A1aY1
Plasmid#158754PurposeExpression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsert6xHistidine_TagRFP-T_A1aY1
Tags6x-Histidine tag and TagRFP-TExpressionMammalianPromoterCMV PromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-GAlphai2-RLuc8
Plasmid#140974PurposeEncodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai2-Rluc8 (GNAI2 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWZL Neo Myr Flag AAK1
Plasmid#20133DepositorPublicationAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL C162F-pRc/CMV
Plasmid#22042DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
TagsHA-tagExpressionMammalianMutationpoint mutant, converting aa 162 from Cysteine to …Available sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1
Plasmid#162566PurposeUsed for the dual color labeling of cPLA2 and LaminB1 expression.DepositorTagsEGFP, P2A, and mKate2ExpressionMammalianPromoterCMV and SV40Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-FnCas9GFP
Plasmid#130966Purposeexpression of FnCas9-GFP in bacterial cellsDepositorInsertHA-NLS-FnCas9
UseCRISPRTags6xHis-mEGFP-TEV-HA-NLSExpressionBacterialPromoterT7Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCpfSA
Plasmid#187890PurposeCRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureusDepositorTypeEmpty backboneUseCRISPRAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_201
Plasmid#91105PurposeTransformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDVDepositorTypeEmpty backboneExpressionPlantPromoterNo PromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only