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We narrowed to 12,777 results for: NUC

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Showing: 10021 - 10050 of 12777 results

  2. pX330-Flag-HeFSpCas9-H840A

    Plasmid
    #80456
    Purpose
    high fidelity enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HeFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80456-plasmid-map-sequence-id-151478

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  3. PP-NOG1

    Plasmid
    #25113
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PFF0625w:C13732 (PFF0625w Plasmodium falciparum)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pICSL30044

    Plasmid
    #245549
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Nuclear Export Signal (MELALKLAGLM)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245549-plasmid-map-sequence-id-495545

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  5. ACT1-CUP1 9bp dist

    Plasmid
    #234131
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234131-plasmid-map-sequence-id-466522

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  6. ACT1-CUP1 12bp dist

    Plasmid
    #234130
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234130-plasmid-map-sequence-id-466519

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  7. ACT1-CUP1 27bp dist

    Plasmid
    #234129
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234129-plasmid-map-sequence-id-466539

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  8. ACT1-CUP1 15bp dist

    Plasmid
    #234128
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234128-plasmid-map-sequence-id-466529

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  9. ACT1-CUP1 42bp dist

    Plasmid
    #234127
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234127-plasmid-map-sequence-id-466531

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  10. ACT1-CUP1 50bp dist

    Plasmid
    #234112
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234112-plasmid-map-sequence-id-466054

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  11. ACT1-CUP1 46bp dist

    Plasmid
    #234111
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234111-plasmid-map-sequence-id-466051

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  12. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid
    #221540
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221540-plasmid-map-sequence-id-437823

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  13. KBB258

    Plasmid
    #185072
    Purpose
    Bacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusion
    Depositor
    Ken Belanger
    Insert
    NUP1
    Tags
    GST
    Expression
    Yeast
    Mutation
    Nup1 N-term+FXFG repeats
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185072-plasmid-map-sequence-id-368132

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  15. DsRed-CPI-17-T38A-NLS

    Plasmid
    #100792
    Purpose
    Mammalian expression of Fluorescent CPI-17 neutral mutation nuclear
    Depositor
    Joseph Gordon
    Insert
    protein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A Human)
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    100792-plasmid-map-sequence-id-193236

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  16. pBS2ndd

    Plasmid
    #97353
    Purpose
    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, ampicillin resistant
    Depositor
    Jamorn Somana
    Insert
    Nucleoid Disruptive Domain
    Use
    Cloning
    Expression
    Bacterial
    Mutation
    changed methionine 1 to isoleucine
    Promoter
    E. coli lacZ promoter
    Available Since
    Aug. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    97353-plasmid-map-sequence-id-190845

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  17. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

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  18. lenti-dCas9-VP64-BFP

    Plasmid
    #196711
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-VP64-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196711-plasmid-map-sequence-id-392561

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  19. EBFP2-H2B-6

    Plasmid
    #55243
    Purpose
    Localization: Nucleus/Histones, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55243-plasmid-map-sequence-id-159436

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  20. mCherry-H2A-10

    Plasmid
    #55054
    Purpose
    Localization: Nucleus/Histones, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    H2A (H2AC13 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55054-plasmid-map-sequence-id-421913

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  21. EGFP-H2B-6

    Plasmid
    #56436
    Purpose
    Localization: Nucleus/Histones, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56436-plasmid-map-sequence-id-275092

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  22. ZsGreen1-H2B-6

    Plasmid
    #56514
    Purpose
    Localization: Nucleus/Histones, Excitation: 493, Emission: 505
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    ZsGreen1
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Promoter
    Mouse Hsp68 minimal promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170102-plasmid-map-sequence-id-346308

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  24. Cre Shine

    Plasmid
    #37404
    Purpose
    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.
    Depositor
    Thomas Hughes
    Insert
    Cre Shine
    Use
    Cre/Lox
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    37404-plasmid-map-sequence-id-114840

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  25. Clover2-H2B-6

    Plasmid
    #56536
    Purpose
    Localization: Nucleus/Histones, Excitation: 505, Emission: 515
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    Clover2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56536-plasmid-map-sequence-id-269078

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  26. pHR-mCh-Cry2WT_NLS

    Plasmid
    #221925
    Purpose
    Expression of SV40 nuclear localization signal (NLS) fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    SV40 NLS
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of SV40 NLS
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221925-plasmid-map-sequence-id-437713

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  27. mTagYFP-H2B-6

    Plasmid
    #56578
    Purpose
    Localization: Nucleus/Histones, Excitation: 508, Emission: 524
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    mTagYFP
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56578-plasmid-map-sequence-id-226596

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  28. lenti-UCOE-dCas9-BFP-VP64

    Plasmid
    #196717
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196717-plasmid-map-sequence-id-388038

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  29. mEmerald-H3-23

    Plasmid
    #54115
    Purpose
    Localization: Nucleus/Histones, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    H3 (H3C2 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54115-plasmid-map-sequence-id-260199

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  30. BFP-RanQ69L

    Plasmid
    #163438
    Purpose
    Mammalian expression of RanQ69L with tag-BFP; contains miniAux domain for degredation
    Depositor
    Sanford Simon
    Insert
    RanQ69L (RAN Human)
    Tags
    tag-BFP
    Expression
    Mammalian
    Mutation
    Q69L
    Promoter
    CMV
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163438-plasmid-map-sequence-id-321655

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Showing: 10021 - 10050 of 12777 results