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We narrowed to 16,181 results for: nes

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Showing: 6681 - 6700 of 16181 results

  1. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid
    #192438
    Purpose
    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    Tags
    Full-length LAMP1 and mCherry
    Expression
    Mammalian
    Mutation
    Rheb Cys 181 mutated to Ser; abolishes C-terminal…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192438-plasmid-map-sequence-id-379134

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  2. pcDNA3-mCherry-Rheb(C181S)

    Plasmid
    #192437
    Purpose
    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Rheb(C181S) (RHEB Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Rheb Cys 181 mutated to Ser; abolishes C-terminal…
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192437-plasmid-map-sequence-id-379127

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  3. miniCGBE1-VRQR (pBM1173)

    Plasmid
    #140255
    Purpose
    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE4max(R33A)_VRQR-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140255-plasmid-map-sequence-id-291776

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  5. pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

    Plasmid
    #107323
    Purpose
    Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-NmCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 (R1335K) is fused to nuclease dead NmCas9 …
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107323-plasmid-map-sequence-id-204717

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  6. pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991)

    Plasmid
    #61848
    Purpose
    for making stable RPE cells expressing WT cdc20
    Depositor
    Jonathon Pines
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cdc20 (CDC20 Human)
    Tags
    Flag and Venus
    Expression
    Mammalian
    Mutation
    wobbled
    Promoter
    CMV
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. DF-UAS-spa-GCaMP6f

    Plasmid
    #67568
    Purpose
    Zebrafish expression of a photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Publication
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    spa-GCaMP6f
    Use
    Zebrafish transgenesis (see :a spinal opsin contr…
    Tags
    HIS-tag
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Available since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pDual-eGFP(H66)

    Plasmid
    #63558
    Purpose
    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Publication
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(H66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pPD292 PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-BFP

    Plasmid
    #249156
    Purpose
    Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP with HBB IVS2 intron in PPH coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Publication
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-mTagBFP2 (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249156-plasmid-map-sequence-id-496442

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  19. pCOCK CM-C02

    Plasmid
    #46563
    Purpose
    Mammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.
    Insert
    CMV mutant promoter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    Mutant version of CMV promoter (refer to Table 1 …
    Available since
    Sept. 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pCOCK CM-C18

    Plasmid
    #46560
    Purpose
    Mammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.
    Insert
    CMV mutant promoter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    Mutant version of CMV promoter (refer to Table 1 …
    Available since
    Sept. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 6681 - 6700 of 16181 results