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We narrowed to 14,812 results for: nts

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Showing: 10051 - 10080 of 14812 results

  2. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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  5. pKLV-EF1aGFP2AKAT7-linker-mAID-W

    Plasmid
    #159294
    Purpose
    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, mAID-tagged) driven by human EF1a promoter
    Depositor
    Kosuke Yusa
    Article
    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:
    Insert
    KAT7 (KAT7 Human)
    Use
    Lentiviral
    Tags
    mAID (auxin-inducible degron)-tagged
    Expression
    Mammalian
    Mutation
    CRISPR sites mutated (gRNA ID. 5 and A10)
    Promoter
    human EF1a promoter
    Available Since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    159294-plasmid-map-sequence-id-313584

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  8. pCEP4-myc-S (B.1.351 lineage / South African variant)

    Plasmid
    #169849
    Purpose
    Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tag
    Depositor
    Erik Procko
    Article
    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d
    Insert
    Protein S
    Tags
    HA leader and c-myc tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    169849-plasmid-map-sequence-id-329351

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  27. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106185
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Has Service
    AAV1
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    hSynapsin
    Available Since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106185-plasmid-map-sequence-id-204585

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  30. pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139092
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9 3.7-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139092-plasmid-map-sequence-id-273889

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Showing: 10051 - 10080 of 14812 results