We narrowed to 11,282 results for: Vars;

1. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

   Plasmid

   #192374

   Purpose

   To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCStrunc-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAFMW-Nbr[K216E]

   Plasmid

   #188600

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   K216E [AAA=> GAA]

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAFMW-Nbr[C399-625 CM]

   Plasmid

   #188588

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler C-terminal domain, catalytic mutant
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   D435A [GAT=>GCC]; E435A [GAA=>GCC]

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAFMW-Nbr[C399-625]-Gwpep(Mm)

   Plasmid

   #188589

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc and GWpep mm

   Expression

   Insect

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAFMW-Nbr[K276A]

   Plasmid

   #188597

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   K276A [AAG=>GCG]

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAFMW-Nbr[C399-625]-Gwpep(Dm)

   Plasmid

   #188590

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc and Gwpep dm

   Expression

   Insect

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAFMW-Gwpep(Dm)-Nbr[C399-625]

   Plasmid

   #188592

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc-Gwpep dm

   Expression

   Insect

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAFMW-Gwpep(Mm)-Nbr[C399-625]

   Plasmid

   #188591

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc-Gwpep mm

   Expression

   Insect

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAFMW-Nbr[R272A,K276A]

   Plasmid

   #188596

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   R272A [CGG=> GCG], K276A [AAG=>GCG]

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAFMW-Nbr[K284A]

    Plasmid

    #188595

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K284A [AAG=> GCG]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAFMW-Nbr[R272A]

    Plasmid

    #188594

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAFMW-Nbr[K187A]

    Plasmid

    #188593

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K187A [AAA=> GCA]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAFMW-Nbr

    Plasmid

    #188584

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

    Plasmid

    #158771

    Purpose

    Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)
    

    Use

    Lentiviral

    Mutation

    Flexible linkers, ADVVCC/QMSY cleavage site

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid

    #181976

    Purpose

    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

    Depositor

    Jan-Henning Klusmann

    Inserts

    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFX-Sirius-KRasCT

    Plasmid

    #175831

    Purpose

    Express Sirius with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with plasma membrane-targeting sequence
    

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFX-SECFP-KRasCT

    Plasmid

    #175832

    Purpose

    Express SECFP with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    SECFP with plasma membrane-targeting sequence
    

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFX-iRFP-KRasCT

    Plasmid

    #175836

    Purpose

    Express iRFP with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with plasma membrane-targeting sequence
    

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSUMO human-cPLA2-C2-domain

    Plasmid

    #170752

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Human)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30 W96A-GLTP

    Plasmid

    #170734

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

    Insert

    W96A-GLTP (GLTP Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    W96A

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMJB1-RPL1B-Cl

    Plasmid

    #160430

    Purpose

    Expresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Insert

    RPL1B
    

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Removed introns, shuffled codons to vary nucleoti…

    Promoter

    S. cerevisiae GAL1

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET15-His-∆NGlpG(Y205F)

    Plasmid

    #155132

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y205F, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MYMH 351

    Plasmid

    #138058

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-10AG 11AC-GFP
    

    Expression

    Bacterial

    Mutation

    AAA GAG GAG GCA

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MYMH 336

    Plasmid

    #138057

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 2AC 11AC-GFP
    

    Expression

    Bacterial

    Mutation

    GCA GAG GAG ACA

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MYMH 333

    Plasmid

    #138056

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 11AC-GFP
    

    Expression

    Bacterial

    Mutation

    GAA GAG GAG ACA

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MYMH 330

    Plasmid

    #138055

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb- 2AC 11AC-GFP
    

    Expression

    Bacterial

    Mutation

    ACA GAG GAG ACA

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MYMH 327

    Plasmid

    #138054

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 2AC-GFP
    

    Expression

    Bacterial

    Mutation

    GCA GAG GAG AAA

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET20b-YbAnbu

    Plasmid

    #129637

    Purpose

    Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbAnbu
    

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    V14A and G107H

    Promoter

    T7

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET20b-YbAnbuT1A

    Plasmid

    #129638

    Purpose

    Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbAnbuT1A
    

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    T1A, V14A and G107H

    Promoter

    T7

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET41a iGluSnFR E25R

    Plasmid

    #119831

    Purpose

    Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25R
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25R

    Promoter

    T7

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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