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Showing: 6721 - 6740 of 19450 results

  2. pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

    Plasmid
    #69842
    Purpose
    This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag
    Depositor
    Michel Bornens
    Publication
    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PLK4 (PLK4 Human)
    Tags
    3xFLAG tag and EGFP
    Expression
    Mammalian
    Mutation
    K41M-S285A-T289A
    Promoter
    CMV
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69842-plasmid-map-sequence-id-123881

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  3. pmiR-96 cluster promoter

    Plasmid
    #62517
    Purpose
    microRNA 183-96-182 cluster promoter luciferase plasmid
    Depositor
    Bharat Ramratnam
    Publication
    Tang et al Nucleic Acids Res. 2014 Mar;42(5):2988-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    promoter of microRNA-183-96-182 cluster
    Tags
    RenSP (optimized renilla luciferase gene)
    Expression
    Mammalian
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. p1.1-mCherry

    Plasmid
    #162772
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Publication
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    red fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162772-plasmid-map-sequence-id-339102

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  8. p1.2-GS-eGFP

    Plasmid
    #162771
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Publication
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162771-plasmid-map-sequence-id-339094

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  9. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

    Plasmid
    #172908
    Purpose
    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
    Depositor
    GENIE Project
    Publication
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Has service
    AAV1
    Insert
    Ace2N-4AA-mNeon-ST A122D
    Use
    AAV and Cre/Lox
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    hSynapsin1
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172908-plasmid-map-sequence-id-341477

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  12. Venus2-Y705F-STAT3

    Plasmid
    #123173
    Purpose
    Expresses Y705F STAT3 fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    Tags
    Venus2 (aa 158-238) for bimolecular fluorescence …
    Expression
    Mammalian
    Mutation
    Y705F substitution
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123173-plasmid-map-sequence-id-238654

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  13. Venus2-S727A-STAT3

    Plasmid
    #123175
    Purpose
    Expresses S727A STAT3 fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    Tags
    Venus2 (aa 158-238) for bimolecular fluorescence …
    Expression
    Mammalian
    Mutation
    S727A substitution
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123175-plasmid-map-sequence-id-238640

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  14. pSCIP-hCD69-TdTomato

    Plasmid
    #154095
    Purpose
    Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene
    Depositor
    Scott McComb
    Publication
    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    [5HA]-P2A-tdTomato-[3HA]
    hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    154095-plasmid-map-sequence-id-307109

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  15. pGBW-m4092686

    Plasmid
    #153895
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Bacterial
    Mutation
    Nearly native encoding;inactivating mutations in …
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153895-plasmid-map-sequence-id-307318

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  16. pGBW-m4046879

    Plasmid
    #149238
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149238-plasmid-map-sequence-id-357573

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  17. pGBW-m4046809

    Plasmid
    #145685
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145685-plasmid-map-sequence-id-297845

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  18. pGBW-m4046933

    Plasmid
    #145592
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145592-plasmid-map-sequence-id-292573

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  20. pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA

    Plasmid
    #104984
    Purpose
    Expresses ST-Renilla reporter in mammalian cells
    Depositor
    Jeffrey Chao
    Publication
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Renilla luciferase
    Tags
    4xGCN4 repeats (SunTag), 24xMS2v5 stem loops in 3…
    Expression
    Bacterial and Mammalian
    Promoter
    TetCMV
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104984-plasmid-map-sequence-id-202898

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Showing: 6721 - 6740 of 19450 results