We narrowed to 10,337 results for: plasmids 101

1. LLP453 pEf1a-NLS-aGCN4-mCherry

   Plasmid

   #100945

   Purpose

   mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, mCherry
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PTPRA-MAC

   Plasmid

   #139632

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   PTPRA (PTPRA Human)
   

   Use

   Tags

   StrepIII-HA-BirA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRE-UBC-hCD46-T2A-GFP

   Plasmid

   #205457

   Purpose

   lentiviral plasmid for expression of human CD46

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD46 (CD46 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

   Plasmid

   #197885

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV2-miniSOG-VAMP2-T2A-mCherry

   Plasmid

   #50970

   Purpose

   AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

   Depositor

   Roger Tsien

   Article

   Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

   Insert

   miniSOG-VAMP2-T2A-mCherry (Vamp2 Arabdopsis thaliana, Mouse)
   

   Use

   AAV

   Tags

   T2A-mCherry and miniSOG

   Expression

   Mutation

   Promoter

   human synapsin promoter

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-SYN_mEGFP-CREB

   Plasmid

   #137006

   Purpose

   AAV backbone, mEGFP-CREB donor for G-CREB

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

   Insert

   CREB1 (Creb1 Rat)
   

   Use

   AAV

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC414 - pAAV EF1a hPREP

   Plasmid

   #59967

   Purpose

   An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn-Aion-citrine

   Plasmid

   #192569

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Article

   Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

   Insert

   Aion
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

   Plasmid

   #197893

   Purpose

   Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   EF1a

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEX-SaCas9-U6-sgGrin2a

    Plasmid

    #124850

    Purpose

    Mutagenesis of Grin2a

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2a (Grin2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRiboI-Sec-APEX2m

    Plasmid

    #176845

    Purpose

    Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

    Depositor

    Jessica Seeliger

    Article

    Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

    Insert

    pRibo-Sec-APEX2m
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRibo, theophilline riboswitch inducible hsp60 pr…

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

    Plasmid

    #124860

    Purpose

    Mutagenesis of Slc17a6

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a6 (Slc17a6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-SYN_mCyRFP2-CREB

    Plasmid

    #137008

    Purpose

    AAV backbone, mCyRFP2-CREB donor for R-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    AAV

    Tags

    mCyRFP2

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD/Paro-IFN

    Plasmid

    #205439

    Purpose

    A donor vector plasmid for Cre-mediated integration of paromomycin resistance (Paro) and dog IFNα-4 (IFNα-4) genes

    Depositor

    Masamichi Kamihira

    Article

    Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

    Inserts

    dog interferon α-4 gene
    paromomycin resistance gene
    

    Use

    Cre/Lox; Chlamydomonas expression

    Tags

    Expression

    Mutation

    Promoter

    Heat shock protein 70A (HSP70A)/ribulose bisphosp…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgRNA1_ASCL1

    Plasmid

    #64129

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDK637

    Plasmid

    #134203

    Purpose

    bacterial expression of human CENP-N 1-240

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP-N 1-240-His (CENPN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-TetOminiCMV-Sox2

    Plasmid

    #136612

    Purpose

    Dox-inducible lentiviral vector expressing mouse Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSyn-Aion-ts-citrine-ER

    Plasmid

    #192575

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Article

    Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

    Insert

    Aion
    

    Use

    AAV

    Tags

    ts-Citrine-ER

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)

    Plasmid

    #129667

    Purpose

    A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. FMR1-E17C-3xFLAG

    Plasmid

    #157784

    Purpose

    donor plasmid for C-terminal FLAG tag fusion of human FMR1

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1-FLAG (FMR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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