We narrowed to 11,684 results for: cel.2

1. hA3A*-IscBn

   Plasmid

   #227146

   Purpose

   Vector encoding human codon-optimized nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter

   Depositor

   Dali Li

   Article

   Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

   Insert

   bpNLS-hA3A*-linker-nOgeuIscB-nucleoplasmin NLS-2XUGI-bpNLS
   

   Use

   Tags

   Expression

   Mutation

   D96R/E84R/V159R/H339A

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TadA-8e-eIscBn-N-HMG-D

   Plasmid

   #227143

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in N terminus driven by CMV promoter

   Depositor

   Dali Li

   Article

   Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

   Insert

   bpNLS-HMG-D-linker-TadA-8e-linker-nOgeuIscB-v3-bpNLS
   

   Use

   Tags

   Expression

   Mutation

   D96R/E84R/V159R/H339A

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIG-hNFATc2

   Plasmid

   #74050

   Purpose

   retroviral expression plasmid for human NFATc2/C

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc2, isoform C (NFATC2 Human)
   

   Use

   Retroviral

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   silent mutation A1723C in the sequence of NM_1730…

   Promoter

   pMSCV-LTRs

   Available since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

   Plasmid

   #182049

   Purpose

   expression of Sox2 mutants

   Depositor

   Hyonchol Jang

   Article

   Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S248A/T258A

   Promoter

   CAG

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag‐mTim3 (T1 truncation)

   Plasmid

   #49210

   Purpose

   expresses Flag tagged mouse Tim3 (T1 truncation)

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 T1 truncation (Havcr2 Mouse)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   T1 truncation ( contains all but the three most C…

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD649-HAsp-SEMA3D-COMP5AP-AviTag-9xHis

   Plasmid

   #157530

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA3D (SEMA3D Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-SaPE2-P2A-BSD

   Plasmid

   #174818

   Purpose

   Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   SaPE2-P2A-BSD
   

   Use

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag‐mTim3 (del.Cyto)

   Plasmid

   #49209

   Purpose

   expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 (del.Cyto) (Havcr2 Mouse)
   

   Use

   Tags

   CD8 signal sequence and FLAG

   Expression

   Mammalian

   Mutation

   cytosolic domain deleted, no Tim3 signal sequence…

   Promoter

   Ef1a

   Available since

   Dec. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag‐mTim3 (T2 truncation)

   Plasmid

   #49211

   Purpose

   expresses Flag tagged mouse Tim3 (T2 truncation)

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 T2 truncation (Havcr2 Mouse)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   T2 truncation ( lacks all but one tyrosine, which…

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Donor_YTHDF2_KO_Hygro

    Plasmid

    #186671

    Purpose

    Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Hygromycin (YTHDF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Donor_YTHDF2_KO_Puro

    Plasmid

    #186670

    Purpose

    Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Puromycin (YTHDF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMPUW-miR-124-GFP-Puro

    Plasmid

    #117321

    Purpose

    Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    hsa-miR-124-1 (MIR124-1 Human)
    

    Use

    Lentiviral

    Tags

    GFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE2-P2A-BSD

    Plasmid

    #174819

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-BSD
    

    Use

    Tags

    P2A-BSD and SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-tetpA(hBCL2-miR9/9*-124)-iRPT

    Plasmid

    #154881

    Purpose

    Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Inserts

    iRFP713
    Puromycin resistance gene
    rtTAM2
    BCL2 (BCL2 Human)
    miR9/9*-124
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIS1 INSIG2

    Plasmid

    #60798

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and wild-type miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    INSIG2 3'UTR and wild-type miR-155 binding site (INSIG2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIS1 mINSIG2

    Plasmid

    #60799

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and mutated miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    INSIG2 3'UTR and mutated miR-155 binding site (INSIG2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-155 binding site

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Donor_YTHDF2_KO_Neo

    Plasmid

    #186672

    Purpose

    Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Neomycin (YTHDF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-tetO-NB

    Plasmid

    #197092

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. DNA-CARzeta-GFP

    Plasmid

    #89344

    Purpose

    2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    DNA-CARzeta (CD247, CD86 Human)
    

    Use

    Lentiviral

    Tags

    SNAPf tag and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HcKCR2_mCherry_pcDNA3.1

    Plasmid

    #177337

    Purpose

    Expresses KCR2 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR2
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mammalian codon-adapted

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCEP4_WT_OCT4

    Plasmid

    #40629

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pMSCV-Myc-Caspase-4_W267N

    Plasmid

    #214311

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    W267N

    Promoter

    MESV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOIMars1CyN

    Plasmid

    #75221

    Purpose

    Mammalian cell surface expression of fluorogen activating protein Mars1Cy

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    ss-HA-Mars1Cy-CD80(237-306)
    

    Use

    Tags

    HA epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCNL-N1_PTS2

    Plasmid

    #65676

    Purpose

    Expresses PTS2-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    peroxisomal targeting signal 2
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pONL-N1_PTS2

    Plasmid

    #65677

    Purpose

    Expresses PTS2-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    peroxisomal targeting signal 2
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pZac2.1-GfaABC1D -IgK- TurboID- NCAN ELS-HA

    Plasmid

    #219809

    Purpose

    Secreted HA-tagged TurboID-NCAN-ELS domain for extracellular proximity labeling

    Depositor

    Cagla Eroglu

    Article

    Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

    Insert

    GfaABC1D
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMSCV-Myc-Caspase-4_R269D

    Plasmid

    #214310

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    R269D

    Promoter

    MESV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMSCV-Myc-Caspase-4_K356D

    Plasmid

    #214309

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    K356D

    Promoter

    MESV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYNL-N1_PTS2

    Plasmid

    #65675

    Purpose

    Expresses PTS2-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    peroxisomal targeting signal 2
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-HA-mIFITM1-C103A

    Plasmid

    #58419

    Purpose

    Expresses murine IFITM1-C103A with an N-terminal HA tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

    Insert

    IFITM1 (Ifitm1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Cysteine 103 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only