We narrowed to 13,339 results for: GAN

1. ZJOM149

   Plasmid

   #133646

   Purpose

   Integration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-ELO3-mCherry (ELO3 Budding Yeast)
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6M-Pak1 K299R

   Plasmid

   #12210

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Catalytically inactive Pak1: K299R

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUCXMG

   Plasmid

   #204409

   Purpose

   Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

   Depositor

   David Ackerley

   Article

   Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry N1 TOMM22

   Plasmid

   #157762

   Purpose

   Expression of TOMM22:mcherry in cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   TOMM22 (TOMM22 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-TRE-SHH

   Plasmid

   #134350

   Purpose

   Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

   Depositor

   Lorenz Studer

   Article

   Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

   Insert

   SHH (SHH Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2 8xSTAR-mScarletI-NLS. PGK-puro

   Plasmid

   #136263

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mScarletI-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

   Plasmid

   #87694

   Purpose

   Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   ERT2-PhoCl-Cre-PhoCl-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. ZJOM65

   Plasmid

   #133654

   Purpose

   Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPH1-mCherry (VPH1 Budding Yeast)
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUASTattB-dALFANb(5mut)-mGreenLantern

   Plasmid

   #219661

   Purpose

   Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-MLS-KillerRed

    Plasmid

    #115517

    Purpose

    Middle entry plasmid containing membrane bound KillerRed fluorophore

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. human STIM1 CFP

    Plasmid

    #19755

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-RAB-AKARev

    Plasmid

    #118478

    Purpose

    ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-AKARev
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. NFAST(low)-mApple-mito

    Plasmid

    #214415

    Purpose

    Mitochondria localization of low-affinity NFAST fused to mApple-mito

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Use

    Tags

    mApple-mito

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-EF1a-Ngn3ER-PuroR-mCherry

    Plasmid

    #197385

    Purpose

    To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

    Depositor

    Qiao Zhou

    Article

    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

    Insert

    Ngn3ER-2A-PuroR-2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. hSTARR-seq_SCP1 vector_blocking 4

    Plasmid

    #99319

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs-IP-PEX3-EGFP

    Plasmid

    #228142

    Purpose

    Expresses PEX3-GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    PEX3 (PEX3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB_iMYOD1_P2A_BAF60C2_T2A_mCh_Puro

    Plasmid

    #168807

    Purpose

    PiggyBac transposon construct with doxycycline inducible MYOD1, BAF60C + mCherry expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    MYOD1 (MYOD1 Synthetic, Human)
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-Flavidin

    Plasmid

    #89881

    Purpose

    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K80R, K121R, K132R, K134R

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-sapphireCKAR

    Plasmid

    #118468

    Purpose

    cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTC391

    Plasmid

    #91220

    Purpose

    protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNA targeting tomato ANT1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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