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We narrowed to 5,097 results for: puc

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Showing: 1651 - 1700 of 5097 results

  2. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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  3. pAAV-hsynapsin-DIO-adra2a-shRNA-mCherry

    Plasmid
    #245063
    Purpose
    AAV-transgene knocking down adra2a receptor (adrenergic 2a receptor) transcripts cell type-selectively. Using the pPRIME. system, this generates mir30-derived shRNAs and a marker from the same RNA.
    Depositor
    William Wisden
    Article
    Anuncibay Soto et al Proc Natl Acad Sci U S A. 2025 Oct 14;12
    Insert
    AAV-human synapsin promoter-DIO-adra2a-shRNA-mCherry (Adra2a Synthetic, Mouse)
    Use
    AAV, Cre/Lox, and RNAi
    Promoter
    human synapsin
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    245063-plasmid-map-sequence-id-489355

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  10. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

    Plasmid
    #229678
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    monomeric StayGold, HA
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229678-plasmid-map-sequence-id-457125

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  12. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid
    #229679
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    monomeric StayGold
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229679-plasmid-map-sequence-id-457128

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  13. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid
    #229680
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    SNAP, V5
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229680-plasmid-map-sequence-id-457075

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  14. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229676-plasmid-map-sequence-id-457076

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  15. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

    Plasmid
    #229677
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    SNAP, 3xV5
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229677-plasmid-map-sequence-id-457129

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  16. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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  18. Bxb1-GT-Dox-AIO-TetOn_Ngn2-ISL1-LHX3_Down-Tandem

    Plasmid
    #241396
    Purpose
    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of Ngn2, ISL1 and LHX3
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    TRE-Ngn2-ISL1-LHX3; rtTA-T2A-mTagBFP2 (ISL1, LHX3, NEUROG2 Human)
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    TRE; CAG
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    241396-plasmid-map-sequence-id-489344

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  19. pHybr_r2a>Fab-srt-his

    Plasmid
    #124810
    Purpose
    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    rat IgG2a Hinge-G4S-Sortag-Histag
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial
    Promoter
    pUC
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124810-plasmid-map-sequence-id-243698

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  23. pminTolR4-R2

    Plasmid
    #67711
    Purpose
    mini Tol2 backbone with att4-attR2 cassette
    Depositor
    Nathan Lawson
    Type
    Empty backbone
    Available Since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67711-plasmid-map-sequence-id-117439

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  24. pJKW0457

    Plasmid
    #107586
    Purpose
    AtU6-26 sgRNA
    Depositor
    Jing-Ke Weng
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    AtU6-26
    Available Since
    April 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    107586-plasmid-map-sequence-id-208233

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  25. AA628

    Plasmid
    #245316
    Purpose
    Fragmid fragment: (CROPseq) cloning with mod1 LTR
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245316-plasmid-map-sequence-id-487913

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  26. pILGFP1DFB

    Plasmid
    #185871
    Purpose
    A parental plasmid for in vivo gene amplification at RPL25 locus
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185871-plasmid-map-sequence-id-370815

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  27. pILGFP3AA5

    Plasmid
    #185875
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185875-plasmid-map-sequence-id-369502

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  28. pILGFP3AG4

    Plasmid
    #185874
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185874-plasmid-map-sequence-id-370818

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  29. pILGFP5EA4

    Plasmid
    #185879
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185879-plasmid-map-sequence-id-370824

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  30. pILGFP5EC4

    Plasmid
    #185880
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185880-plasmid-map-sequence-id-370826

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  31. pILGFP5EF3

    Plasmid
    #185881
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185881-plasmid-map-sequence-id-370828

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  32. pMT1004

    Plasmid
    #8613
    Depositor
    Robert Hartley
    Insert
    binase, barstar
    Expression
    Bacterial
    Mutation
    as pMT1002
    Available Since
    Jan. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    8613-plasmid-map-sequence-id-240677

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  33. AA692

    Plasmid
    #245318
    Purpose
    Fragmid fragment: (Selection) for custom ORF cloning
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    245318-plasmid-map-sequence-id-490102

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  34. pILGFP4BG6

    Plasmid
    #185877
    Purpose
    A parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locus
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185877-plasmid-map-sequence-id-370820

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  35. pILGFP3AE4

    Plasmid
    #185873
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185873-plasmid-map-sequence-id-370817

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  36. AA593

    Plasmid
    #245314
    Purpose
    Fragmid fragment: (2A Selection) mTagBFP2
    Depositor
    John Doench, David Root
    Insert
    mTagBFP2
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    245314-plasmid-map-sequence-id-488937

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  37. AA627

    Plasmid
    #245315
    Purpose
    Fragmid fragment: (CROPseq) cloning with mod1 LTR; iBar-compatible
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245315-plasmid-map-sequence-id-487877

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  38. pILGFP5EG3

    Plasmid
    #185878
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185878-plasmid-map-sequence-id-370821

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  39. pILGFP3A5C

    Plasmid
    #185872
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185872-plasmid-map-sequence-id-370816

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  40. pDmut06

    Plasmid
    #65945
    Purpose
    Theophylline riboswitch D mut derivatives 06
    Depositor
    Justin Gallivan
    Insert
    luciferase
    Expression
    Bacterial
    Available Since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  41. pDmut10

    Plasmid
    #65946
    Purpose
    Theophylline riboswitch D mut derivatives 10
    Depositor
    Justin Gallivan
    Insert
    luciferase
    Expression
    Bacterial
    Available Since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  42. NK690

    Plasmid
    #120849
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Insert
    Jumble-mWasabi
    Use
    Choanoflagellate expression
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120849-plasmid-map-sequence-id-233698

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  43. AA662

    Plasmid
    #245317
    Purpose
    Fragmid fragment: (Promoter) CAG promoter
    Depositor
    John Doench, David Root
    Insert
    CAG
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    245317-plasmid-map-sequence-id-490094

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  44. AA583

    Plasmid
    #245313
    Purpose
    Fragmid fragment: (promoter) CMV promoter
    Depositor
    John Doench, David Root
    Insert
    CMV
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245313-plasmid-map-sequence-id-487909

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  45. pILGFP3AG4ARSd

    Plasmid
    #185876
    Purpose
    A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185876-plasmid-map-sequence-id-370819

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  46. AA557

    Plasmid
    #245312
    Purpose
    Fragmid fragment: (C' terminus) for C>T base editing, 3xHA tag
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245312-plasmid-map-sequence-id-487893

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  47. AA388

    Plasmid
    #245311
    Purpose
    Fragmid fragment: (N' terminus) TadA-CDd
    Depositor
    John Doench, David Root
    Insert
    TadA-CDd
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245311-plasmid-map-sequence-id-487878

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  48. pDA03

    Plasmid
    #65943
    Purpose
    Theophylline riboswitch D derivatives 03
    Depositor
    Justin Gallivan
    Insert
    luciferase
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. pDA08

    Plasmid
    #65944
    Purpose
    Theophylline riboswitch D derivatives 08
    Depositor
    Justin Gallivan
    Insert
    luciferase
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  50. pMDW139

    Plasmid
    #227336
    Purpose
    Template for in vitro Pol II transcription
    Depositor
    Michelle Wang
    Insert
    Gap DNA
    Expression
    Bacterial
    Promoter
    Plac
    Available Since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227336-plasmid-map-sequence-id-451681

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Showing: 1651 - 1700 of 5097 results