-
Plasmid#192688PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_2
Plasmid#192686PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_3
Plasmid#192687PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
Dox nontargeting shRNA GFP
Plasmid#155290PurposeLentivirus for doxycycline inducible expression of nontargeting shRNA, GFP marker, based on Addgene 11652DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOpen-NotIR (GC)
Plasmid#165507PurposeType II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.DepositorInsertNotI (GC-rich recognition site)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Dox nontargeting shRNA mKate
Plasmid#155292PurposeLentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral nontargeting shRNA RFP i670
Plasmid#155287PurposeLentiviral expression of nontargeting shRNA, RFP i670 expression, based on Addgene 12247DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPHB.1.0-gDNA
Plasmid#132453PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertPHB
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pFG018
Plasmid#62817PurposeBacterial inducible gRNA expression plasmidDepositorInsertgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-67
Plasmid#188671PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-1956
Plasmid#188670PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-1883
Plasmid#188669PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pCfb13220
Plasmid#219895PurposeThe base plasmid of TUNEYALI for TF30DepositorInsertContains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13204
Plasmid#219879PurposeThe base plasmid of TUNEYALI for TF14DepositorInsertContains gRNA targeting TF14 (YALI1_F21261g) and homologous arm matching TF14
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13226
Plasmid#219901PurposeThe base plasmid of TUNEYALI for TF36DepositorInsertContains gRNA targeting TF36 (YALI1_A22330g) and homologous arm matching TF36
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13208
Plasmid#219883PurposeThe base plasmid of TUNEYALI for TF18DepositorInsertContains gRNA targeting TF18 (YALI1_D15792g) and homologous arm matching TF18
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13217
Plasmid#219892PurposeThe base plasmid of TUNEYALI for TF27DepositorInsertContains gRNA targeting TF27 (YALI1_E22758g) and homologous arm matching TF27
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only