Addgene

We narrowed to 4,066 results for: abo

Showing: 661 - 680 of 4066 results

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pTETDuet_WTDHFR_WTTS

Plasmid

#191282

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

WT DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pIYC05

Plasmid

#64742

Purpose

Yeast expression plasmid: acsL641P, ALD6

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Insert

acsL641P, ALD6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pMEG_BB3_YL001

Plasmid

#117825

Purpose

Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_YlGUT1_YlCyc1TT

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtI

Plasmid

#114343

Purpose

crtI in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtI (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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glgP-gap1-

Plasmid

#101838

Purpose

deletes glgP_gap1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of synpcc7942_244_245 operon)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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glgC-

Plasmid

#101842

Purpose

deletes glgC locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of glgC gene)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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glgA-

Plasmid

#101841

Purpose

deletes glgA locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of glgA gene)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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gnd-

Plasmid

#101840

Purpose

deletes gnd locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of gnd gene)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pStA0::eyfp

Plasmid

#114351

Purpose

efyp in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

efyp (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::J23116

Plasmid

#114331

Purpose

Promoter J23116 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Promoter J23116

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::RBSc42

Plasmid

#114338

Purpose

RBS RBSc42 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

RBS RBSc42

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::L3S2P55

Plasmid

#114339

Purpose

Terminator L3S2P55 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Terminator L3S2P55

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtW

Plasmid

#114347

Purpose

crtW in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtW (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMST634-BB3_L_AB_hyg_KanOri_pyrG5

Plasmid

#90279

Purpose

BB3 recipient for homologous integration in pyrG locus with linker AB

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pStA0::crtE

Plasmid

#114345

Purpose

crtE in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtE (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMST636_BB3_L_AD_hyg_KanOri_pyrG5

Plasmid

#90281

Purpose

BB3 recipient for homologous integration in pyrG locus with linker AD

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHPHS309

Plasmid

#228230

Purpose

GFP dropout backbone for sgRNA library cloning

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ0464

Plasmid

#228716

Purpose

Bacterial expression of T7 RNAP with E643G mutation; for protein production and purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

SUmo_T7pol_E643G

Use

Tags

Expression

Bacterial

Mutation

E643G

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBEST-PrOR1OR2-MGapt-UTR1-deGFP

Plasmid

#227648

Purpose

PrOR1OR2-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

MG aptamer (MGapt) and deGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBEST-PTet-MGapt-UTR1-deGFP

Plasmid

#227647

Purpose

pTet-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

MG aptamer (MGapt) and deGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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