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Plasmid#114106Purposefusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_tKiMBI-T2A-caMEK
Plasmid#199578PurposeExpresses tKiMBI and caMEK in an AAV vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin
Plasmid#199585PurposeExpress tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNAZeo(-)HSC73
Plasmid#86030PurposeExpression of Heat shock chaperone 70 in Mammalian cellsDepositorInsertHeat-shock cognate protein 73 (HSPA8 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His6-Mps1-WT
Plasmid#114063Purposeprotein productionDepositorInsertMps1-WT (TTK Human)
UseTagsHis6ExpressionInsectMutationnonePromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
SEP-Nanoluc-cyOFP1
Plasmid#141082Purposeprototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEP-Nanoluc-cyOFP1
UseLentiviralTagsExpressionMutationDeletion of amino acids DISGG from positions 673 …PromoterEF1αAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Hu_Plk5(WT)
Plasmid#136319PurposeMammalian expression of human Plk5-WT with N-terminal EGFPDepositorInsertPLK5 (PLK5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNAZeo(-)HSC73AS
Plasmid#86031PurposeAntisense for pCDNAZeo(-)HSC73DepositorInsertheat shock cognate protein 70 (hsc70) (HSPA8 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-B7-EYFP
Plasmid#114110Purposefusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, slow burn
B7 hinge region
Enhanced Yellow Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nuc-Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71349PurposeExpress Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian celDepositorInsertOrange Nano-lantern(Ca2+)_H2B
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His6-Mps1-KD
Plasmid#114064Purposeprotein productionDepositorInsertMps1-KD (TTK Human)
UseTagsHis6ExpressionInsectMutationMps1 KD (D664A)PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mito-Cyan Nano-lantern(Ca2+)/pcDNA3
Plasmid#71345PurposeExpress Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian celDepositorInserthOTC_Cyan Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_CCDC22(Y557C)
Plasmid#209695PurposeExpresses MAC3-tagged CCDC22(Y557C) in mammalian cellsDepositorInsertCCDC22 (CCDC22 Human)
UseTagsMAC3-tagExpressionMammalianMutationY557C, changed Tyrosine 557 to CysteinePromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_CCDC22(T17A)
Plasmid#209694PurposeExpresses MAC3-tagged CCDC22(T17A) in mammalian cellsDepositorInsertCCDC22 (CCDC22 Human)
UseTagsMAC3-tagExpressionMammalianMutationT17A, changed Threonine 17 to AlaninePromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL0_AC-27877
Plasmid#202110PurposeLevel 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert27877 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI sites fo…PromoterAvailable sinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-Fld
Plasmid#202125PurposeLevel 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertFld promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI site for…PromoterAvailable sinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-54730
Plasmid#202109PurposeLevel 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert54730 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI site for…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-51183
Plasmid#202108PurposeLevel 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert51183 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI sites fo…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-GEF
Plasmid#202107PurposeLevel 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertGEF promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI site for…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-47740
Plasmid#202106PurposeLevel 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert47740 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI site for…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits