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pCMV-FLAG-CAMK2D-ED

Plasmid

#221688

Purpose

Mammalian expression of FLAG-tagged human CAMK2D (K43R/D136N)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CAMK2D (CAMK2D Human)

Use

Tags

Expression

Mammalian

Mutation

K43R, D136N

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT2 NCmut-msfGFP

Plasmid

#180320

Purpose

mammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN2 (SEPTIN2 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT2 F20D, V27D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc F-tractinN9-39-msfGFPΔN7

Plasmid

#231552

Purpose

Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

F-tractin (Itpka Rat)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-CDC20

Plasmid

#221694

Purpose

Mammalian expression of FLAG-tagged human CDC20

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CDC20 (CDC20 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut2

Plasmid

#180425

Purpose

mammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3 Gmut-msfGFP

Plasmid

#180326

Purpose

mammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i3 W502A, H512D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut1

Plasmid

#180316

Purpose

mammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 W269A, H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple-CAAX

Plasmid

#180324

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple and CAAX (HRas)

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3 NCmut-msfGFP

Plasmid

#180325

Purpose

mammalian expression of human SEPT9_i3 NCmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i3 I263D, M270D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry IL36RN S113L

Plasmid

#58321

Purpose

entry vector for human IL36RN S113L mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Expression

Mutation

S113L: c.338C>T, p.Ser113Leu

Promoter

CMV

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pCMV6-Entry IL36RN R102W

Plasmid

#58323

Purpose

entry vector for human IL36RN R102W mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Expression

Mutation

R102W: c.304C>T, p.Arg102Trp

Promoter

CMV

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pCMV6-Entry IL36RN R48W

Plasmid

#58324

Purpose

entry vector for human IL36RN R48W mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Expression

Mutation

R48W: c.142C>T, p.Arg48Trp

Promoter

CMV

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pCMV6-Entry IL36RN P76L

Plasmid

#58322

Purpose

entry vector for human IL36RN P76L mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Expression

Mutation

P76L: c.227C>T,p.Pro76Leu

Promoter

CMV

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pCMV6-Entry IL36RN Κ35R

Plasmid

#58320

Purpose

entry vector for human IL36RN K35R mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Expression

Mutation

Κ35R: c.104A>G, p.Lys35Arg

Promoter

CMV

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-LOx-IRES-EGFP

Plasmid

#166877

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ARMS1-ProLink2-MRGPRX3

Plasmid

#213962

Purpose

Expresses MRGPRX3 in mammalian cells

Depositor

Christa Müller

Article

Al Hamwi et al Commun Biol. 2024 Jan 6;7(1):52. doi: 10

Insert

MRGPRX3 (MRGPRX3 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6M-PAK1 H83L H86L K299R

Plasmid

#26592

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

H83L H86L K299R Dominant negative

Promoter

Available since

March 9, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-PE7-P2A-GFP

Plasmid

#222996

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-EGFP

Use

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-GFP

Plasmid

#180020

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-EGFP

Use

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-hMLH1dn

Plasmid

#174828

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-hMLH1dn

Use

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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